Hung Yung-Li, Ishigami Akihito, Machida Shuichi
Japan Society for the Promotion of Science, 5-3-1 Kojimachi, Chiyoda-ku, Tokyo 102-0083, Japan; Graduate School of Health and Sports Science, Juntendo University, 1-1 Hirakagakuendai, Inzai, Chiba 270-1695, Japan.
Molecular Regulation of Aging, Tokyo Metropolitan Institute for Geriatrics and Gerontology, 35-2 Sakae-cho, Itabashi-ku, Tokyo 173-0015, Japan.
J Physiol Sci. 2025 Mar 27;75(2):100019. doi: 10.1016/j.jphyss.2025.100019.
This study aimed to investigate the influence of aging on steroid hormone production in skeletal muscles in response to resistance training. Male F344 rats, aged 4 months (young) and 22 months (old), were randomized into the sedentary and training groups. The training group performed resistance training by climbing a ladder with a load every three days for eight weeks. After the training period, the flexor hallucis longus muscle was dissection, and muscle steroid hormone levels were analyzed using liquid chromatography-tandem mass spectrometry. We found that resistance training significantly increased muscle mass in young and old rats, although the increase was less pronounced in the latter. In young, trained rats, muscle dihydrotestosterone levels were approximately 35-fold higher compared to sedentary controls (p < 0.01); dihydrotestosterone levels did not differ significantly between sedentary and trained old rats. These findings indicate that resistance training-induced dihydrotestosterone production is blunted in aging rat skeletal muscle.
本研究旨在探讨衰老对骨骼肌中类固醇激素产生的影响，以及其对阻力训练的反应。4个月大（年轻）和22个月大（年老）的雄性F344大鼠被随机分为久坐组和训练组。训练组每三天负重爬梯一次，持续八周进行阻力训练。训练期结束后，解剖拇长屈肌，并用液相色谱-串联质谱法分析肌肉类固醇激素水平。我们发现，阻力训练显著增加了年轻和年老大鼠的肌肉质量，尽管后者的增加不太明显。在年轻的训练大鼠中，肌肉双氢睾酮水平比久坐对照组高约35倍（p < 0.01）；久坐和训练的老年大鼠之间双氢睾酮水平没有显著差异。这些发现表明，在衰老的大鼠骨骼肌中，阻力训练诱导的双氢睾酮产生减弱。
