Snead Anthony A, Meng Fang, Largotta Nicolas, Winchell Kristin M, Levine Brenna A
Department of Biology, New York University, New York, NY, USA.
Center for Genomics & Systems Biology, New York University, New York, NY, USA.
Sci Data. 2025 Apr 5;12(1):579. doi: 10.1038/s41597-025-04854-8.
The spotted lanternfly (Lycorma delicatula) is a planthopper species (Hemiptera: Fulgoridae) native to China but invasive in South Korea, Japan, and the United States where it is a significant threat to agriculture. Genomic resources are critical to both management of this species and understanding the genomic characteristics of successful invaders. We report an annotated, haplotype-phased, chromosome-level genome assembly for the spotted lanternfly using PacBio long-read sequencing, Hi-C technology, and RNA-seq. The 2.2 Gbp genome comprises 13 chromosomes, and whole genome resequencing of eighty-two adults indicated chromosome four as the sex chromosome and a corresponding XO sex-determination system. We identified over 12,000 protein-coding genes and performed functional annotation, facilitating the identification of candidate genes that may hold importance for spotted lanternfly control. The assemblies and annotations were highly complete with over 96% of BUSCO genes complete regardless of the database (i.e., Eukaryota, Arthropoda, Insecta). This reference-quality genome will serve as an important resource for development and optimization of management practices for the spotted lanternfly and invasive species genomics as a whole.
斑衣蜡蝉（Lycorma delicatula）是一种蜡蝉科昆虫（半翅目：蜡蝉科），原产于中国，但已入侵韩国、日本和美国，对当地农业构成重大威胁。基因组资源对于该物种的管理以及了解成功入侵物种的基因组特征至关重要。我们报告了利用PacBio长读长测序、Hi-C技术和RNA测序获得的斑衣蜡蝉的注释、单倍型定相、染色体水平的基因组组装。这个22亿碱基对的基因组由13条染色体组成，对82只成虫进行的全基因组重测序表明，第四条染色体为性染色体，对应的是XO性别决定系统。我们鉴定出超过12000个蛋白质编码基因并进行了功能注释，有助于识别可能对斑衣蜡蝉防治具有重要意义的候选基因。无论使用哪个数据库（即真核生物、节肢动物、昆虫纲），组装和注释的完整性都很高，超过96%的BUSCO基因完整。这个参考质量的基因组将成为开发和优化斑衣蜡蝉管理方法以及整个入侵物种基因组学的重要资源。
