Yorek M A, Figard P H, Kaduce T L, Spector A A
Invest Ophthalmol Vis Sci. 1985 Aug;26(8):1148-54.
A number of differences have been observed in the lipid metabolism of cultured Y79 and WERI Rb-1 retinoblastoma cells. The Y79 cells have a higher phospholipid content and a higher percentage of ethanolamine phosphoglycerides, whereas the WERI Rb-1 cells have more triglycerides. This is consistent with the finding that most of the polyunsaturated fatty acid taken up by the Y79 cells is incorporated into phospholipids, while a sizable amount of the uptake in WERI Rb-1 cells is incorporated into triglyceride. Polyunsaturated fatty acids of the n-6 class are taken up at about the same rate by both cells, but the n-3 polyunsaturates are taken up more rapidly by the Y79 cells. In addition, the Y79 cells convert a larger percentage of linoleic acid (18:2, n-6) to arachidonic acid (20:4, n-6) and of linolenic acid (18:3, n-3) to docosahexaenoic acid (22:6, n-3), and they accumulate these products when the precursors are added to the culture medium. When grown in 10% fetal bovine serum without supplemental fatty acid, however, the WERI Rb-1 contain more arachidonic and docosahexaenoic acids. These differences in lipid content and metabolism suggest that although the Y79 and WERI Rb-1 cell lines are derived from the same type of retinal tumor, they either originated from functionally different retinal neurons or have diverged biochemically during propagation in culture.
在培养的Y79和WERI Rb - 1视网膜母细胞瘤细胞的脂质代谢中观察到了一些差异。Y79细胞具有较高的磷脂含量和较高百分比的乙醇胺磷酸甘油酯，而WERI Rb - 1细胞含有更多的甘油三酯。这与以下发现一致：Y79细胞摄取的大多数多不饱和脂肪酸被整合到磷脂中，而WERI Rb - 1细胞摄取的相当一部分则被整合到甘油三酯中。n - 6类多不饱和脂肪酸在两种细胞中的摄取速率大致相同，但n - 3多不饱和脂肪酸在Y79细胞中的摄取速度更快。此外，Y79细胞将较大比例的亚油酸（18:2，n - 6）转化为花生四烯酸（20:4，n - 6），并将亚麻酸（18:3，n - 3）转化为二十二碳六烯酸（22:6，n - 3），并且当将前体添加到培养基中时它们会积累这些产物。然而，当在不添加补充脂肪酸的10%胎牛血清中生长时，WERI Rb - 1细胞含有更多的花生四烯酸和二十二碳六烯酸。脂质含量和代谢的这些差异表明，尽管Y79和WERI Rb - 1细胞系源自同一类型的视网膜肿瘤，但它们要么起源于功能不同的视网膜神经元，要么在培养繁殖过程中发生了生化分化。
