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兔骨髓中一种内源性凝集素的表面定位。

Surface localization of an endogenous lectin in rabbit bone marrow.

作者信息

Catt J W, Peacock M A, Harrison F L

出版信息

Histochem J. 1985 Feb;17(2):189-99. doi: 10.1007/BF01003218.

DOI:10.1007/BF01003218
PMID:4019249
Abstract

A lectin, which may be involved in cell to cell adhesion during erythropoiesis in rabbit bone marrow, has been isolated and characterized. Several electron microscopical techniques have been used to investigate the cell surface distribution of this lectin in bone marrow utilizing colloidal gold conjugates of anti-lectin IgG or protein A. The lectin is present at the surface of erythroid cells at all stages of development but no lectin was detected on the surface of myeloid cells. The limitations and complementary nature of the techniques used are discussed.

摘要

一种可能参与兔骨髓红细胞生成过程中细胞间黏附的凝集素已被分离和鉴定。利用抗凝集素IgG或蛋白A的胶体金缀合物,采用几种电子显微镜技术研究了这种凝集素在骨髓中的细胞表面分布。凝集素存在于发育各阶段的红系细胞表面,但在髓系细胞表面未检测到凝集素。文中讨论了所用技术的局限性和互补性。

相似文献

1
Surface localization of an endogenous lectin in rabbit bone marrow.兔骨髓中一种内源性凝集素的表面定位。
Histochem J. 1985 Feb;17(2):189-99. doi: 10.1007/BF01003218.
2
Erythroid developmental agglutinin is a protein lectin mediating specific cell-cell adhesion between differentiating rabbit erythroblasts.
Nature. 1980 Jul 31;286(5772):502-4. doi: 10.1038/286502a0.
3
Isoforms of an endogenous lectin in rabbit bone marrow.
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4
[Hybridization of low molecular weight nuclear RNA with pre-mRNA from erythroid bone marrow cells and rabbit mRNA].[低分子量核RNA与红系骨髓细胞前体mRNA及兔mRNA的杂交]
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Separation of haemopoietic cells for biochemical investigation. Preparation of erythroid and myeloid cells from human and laboratory-animal bone marrow and the separation of erythroblasts according to their state of maturation.用于生化研究的造血细胞分离。从人和实验动物骨髓中制备红系和髓系细胞,并根据成红细胞的成熟状态进行分离。
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Quantitation of globin mRNA levels during erythroid development in the rabbit and discovery of a new beta-related species in immature erythroblasts.兔红细胞生成过程中珠蛋白mRNA水平的定量分析以及在未成熟成红细胞中发现一种新的β相关物种。
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Intra- and extracellular distribution of an endogenous lectin during erythropoiesis.
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引用本文的文献

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Silver-enhanced colloidal gold probes as markers for scanning electron microscopy.
Histochemistry. 1986;86(1):35-41. doi: 10.1007/BF00492343.

本文引用的文献

1
Gap junctions between cells of bone marrow: an ultrastructural study using tannic acid.骨髓细胞间的缝隙连接:一项使用鞣酸的超微结构研究
Anat Rec. 1980 Jan;196(1):101-7. doi: 10.1002/ar.1091960110.
2
Separation of haemopoietic cells for biochemical investigation. Preparation of erythroid and myeloid cells from human and laboratory-animal bone marrow and the separation of erythroblasts according to their state of maturation.用于生化研究的造血细胞分离。从人和实验动物骨髓中制备红系和髓系细胞,并根据成红细胞的成熟状态进行分离。
Biochem J. 1981 Mar 15;194(3):789-96. doi: 10.1042/bj1940789.
3
Ultrastructural aspects of erythropoietic differentiation in long-term bone marrow culture.
Differentiation. 1982;21(2):86-94. doi: 10.1111/j.1432-0436.1982.tb01201.x.
4
Factors mediating cell--cell recognition and adhesion. Galaptins, a recently discovered class of bridging molecules.介导细胞间识别与黏附的因子。半乳糖凝集素,一类最近发现的桥接分子。
FEBS Lett. 1980 Dec 29;122(2):157-65. doi: 10.1016/0014-5793(80)80428-5.
5
A review of cell surface markers and labelling techniques for scanning electron microscopy.扫描电子显微镜细胞表面标志物及标记技术综述
Histochem J. 1980 May;12(3):273-315. doi: 10.1007/BF01006952.
6
Erythroid developmental agglutinin is a protein lectin mediating specific cell-cell adhesion between differentiating rabbit erythroblasts.
Nature. 1980 Jul 31;286(5772):502-4. doi: 10.1038/286502a0.
7
Isoforms of an endogenous lectin in rabbit bone marrow.
Eur J Biochem. 1984 Apr 2;140(1):137-41. doi: 10.1111/j.1432-1033.1984.tb08076.x.
8
Air-drying of human leucocytes for scanning electron microscopy using the GTGO procedure.使用GTGO程序对人白细胞进行空气干燥以用于扫描电子显微镜检查。
J Microsc. 1983 Jul;131(Pt 1):87-95. doi: 10.1111/j.1365-2818.1983.tb04234.x.
9
Internalization and processing of transferrin and the transferrin receptor in human carcinoma A431 cells.人癌A431细胞中转铁蛋白及转铁蛋白受体的内化与加工
J Cell Biol. 1983 Aug;97(2):508-21. doi: 10.1083/jcb.97.2.508.
10
Characterization of developing adult mammalian erythroid cells separated by velocity sedimentation.通过速度沉降分离的成年哺乳动物发育中红细胞的特性分析。
Br J Haematol. 1973 Jan;24(1):7-17. doi: 10.1111/j.1365-2141.1973.tb05722.x.