Goras J T, Gauthier A R
J Assoc Off Anal Chem. 1985 May-Jun;68(3):598-601.
A liquid chromatographic (LC) method is proposed for measuring 0.485-0.970% morantel tartrate in cattle feeds. The drug is leached from feed, diluted, separated from interfering substances on a silica column, and measured in the effluent stream by 313 nm spectrophotometric detection. Two potential degradation products, i.e., cis-isomer of morantel tartrate and N-(3-methylaminopropyl)-trans-3-(3-methyl-2-thienyl)acrylamide, and a related anthelmintic, i.e., pyrantel tartrate, do not interfere. Average recovery of drug from liquid spiked samples and laboratory blends was 98-100% with a maximum coefficient of variation (CV) of 2.3%. Results for pelleted and crumbled commercial scale feeds ranged from 94 to 102% of label claim, with a maximum CV of 1.5%.
本文提出了一种液相色谱（LC）法，用于测定牛饲料中0.485 - 0.970%的酒石酸噻嘧啶。该药物从饲料中浸出、稀释，在硅胶柱上与干扰物质分离，并通过313 nm分光光度检测法对流出液进行测定。两种潜在的降解产物，即酒石酸噻嘧啶的顺式异构体和N-(3-甲基氨丙基)-反式-3-(3-甲基-2-噻吩基)丙烯酰胺，以及一种相关的驱虫药，即酒石酸噻嘧啶，均不产生干扰。加标液体样品和实验室混合样品中药物的平均回收率为98 - 100%，最大变异系数（CV）为2.3%。颗粒状和破碎状商业规模饲料的测定结果为标签标注量的94%至102%，最大CV为1.5%。
