通过电子免疫化学法对氧化节杆菌中6-羟基尼古丁氧化酶对映体酶的定位
Localization of the enantiozymes of 6-hydroxy-nicotine oxidase in Arthrobacter oxidans by electron immunochemistry.
作者信息
Swafford J R, Reeves H C, Brandsch R
出版信息
J Bacteriol. 1985 Aug;163(2):792-5. doi: 10.1128/jb.163.2.792-795.1985.
Abstract
During the course of growth of Arthrobacter oxidans, induction of the enantiozymes 6-hydroxy-D-nicotine oxidase and 6-hydroxy-L-nicotine oxidase occurred in the presence of DL-nicotine. Cryoultramicrotomed sections obtained from cells grown to stationary phase were gold immunolabeled. The results obtained demonstrate that both enzymes are localized in the cytoplasm.
摘要
在氧化节杆菌生长过程中，在DL-尼古丁存在的情况下，对映体酶6-羟基-D-尼古丁氧化酶和6-羟基-L-尼古丁氧化酶被诱导产生。从生长至稳定期的细胞获得的冷冻超薄切片进行了金免疫标记。所获得的结果表明这两种酶都定位于细胞质中。
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