Yamaguchi N, Okabe T, Kawai K
J Cancer Res Clin Oncol. 1985;110(1):42-7. doi: 10.1007/BF00402500.
To examine whether a human carcinoembryonic antigen (CEA)-producing cell can proliferate and sythesize CEA in vitro in culture without protein supplements, long-term cultivation of such cells was carried out in a protein-free chemically defined medium. Using stepwise decreases in fetal bovine serum concentration, continuous growth of the cells was established in a protein-free am's F-12 medium. The cells, designated as HLC-Yl, have been propagated in this medium for 3 years. The population doubling time of the cells is about 52 h. Addition of the serum stimulated the cell growth (population doubling time = 27 h). Saturation density was not increased by the addition of serum. The cells grown in a protein-free F-12 secrete large amounts of CEA (65.4 +/- 2.6 ng/10(6) cells/24 h). Addition of serum did not stimulate the production of CEA. The cells produced tumours when inoculated into athymic nude mice. The mice bearing the tumour showed high serum CEA levels, and CEA was demonstrated in the tumour tissue by the immunoperoxidase method. The present study suggests that cells grown in a protein-free medium do not require serum components for their growth or CEA synthesis and provide an excellent model for better understanding the growth and production of CEA in human lung cancer cells.
为了研究产生人癌胚抗原（CEA）的细胞在无蛋白质补充物的体外培养条件下能否增殖并合成CEA，我们在无蛋白质的化学限定培养基中对这类细胞进行了长期培养。通过逐步降低胎牛血清浓度，在无蛋白质的阿姆氏F-12培养基中实现了细胞的持续生长。这些细胞被命名为HLC-Y1，已在该培养基中传代培养3年。细胞的群体倍增时间约为52小时。添加血清可刺激细胞生长（群体倍增时间 = 27小时）。添加血清并未增加饱和密度。在无蛋白质的F-12培养基中生长的细胞分泌大量CEA（65.4±2.6 ng/10⁶细胞/24小时）。添加血清并未刺激CEA的产生。将这些细胞接种到无胸腺裸鼠体内可产生肿瘤。携带肿瘤的小鼠血清CEA水平较高，通过免疫过氧化物酶法在肿瘤组织中检测到了CEA。本研究表明，在无蛋白质培养基中生长的细胞在生长或CEA合成过程中不需要血清成分，为更好地理解人肺癌细胞中CEA的生长和产生提供了一个良好的模型。
