Kanazawa S, Morikawa S, Harada T, Yasuhira K, Matsubara Y, Ikeda S, Soeda S, Fujimura M
Cancer. 1982 Nov 1;50(9):1775-82. doi: 10.1002/1097-0142(19821101)50:9<1775::aid-cncr2820500922>3.0.co;2-t.
Of 16 human malignant tumor cell lines established in our laboratory, seven lines, including three gastric cancer cell lines derived from patients with cancer, were found to carry carcinoembryonic antigen on their cell surface, as determined by radioimmunoassay and indirect immunofluorescence. The existence of antibodies (IgG class) against the gastric cancer cell lines (HPE-GAC-T, -2, -3) and lung cancer cell line (HPL-Ad-K) in the sera of patients with gastrointestinal cancer (incidence 70.8%) was demonstrated by indirect immunofluorescence. In nonmalignant cases and healthy controls, the incidence was 7.7 and 3.2%, respectively. Specificity of the antibodies detected in the sera of patients with gastric cancer was examined by absorption and blocking test methods of immunofluorescence. Even though there was an apparent heterogeneity of the specificity among the antibodies, the detection of such antibodies may be a feasible and practical approach to a clinical diagnosis of malignancy.
在我们实验室建立的16种人类恶性肿瘤细胞系中，通过放射免疫测定和间接免疫荧光法测定，发现其中7种细胞系，包括3种源自癌症患者的胃癌细胞系，在其细胞表面携带癌胚抗原。通过间接免疫荧光法证实，胃肠道癌症患者血清中存在针对胃癌细胞系（HPE-GAC-T、-2、-3）和肺癌细胞系（HPL-Ad-K）的抗体（IgG类）（发生率70.8%）。在非恶性病例和健康对照中，发生率分别为7.7%和3.2%。通过免疫荧光的吸收和阻断试验方法检测了胃癌患者血清中检测到的抗体的特异性。尽管抗体之间的特异性存在明显异质性，但检测此类抗体可能是一种可行且实用的恶性肿瘤临床诊断方法。
