Mi Qianwen, Sun Hang, Wang Heyu, Li Qiuyang, Yin Pei, Wang Qiannan, Tong Jian, Liu Shuang, Bi Yunfeng, Yu Lei
College of Food Science and Engineering, Jilin Agricultural University, Changchun, 130118 China.
Food Sci Biotechnol. 2025 Feb 14;34(9):2011-2024. doi: 10.1007/s10068-025-01817-y. eCollection 2025 May.
The issue of Cervical cancer has received considerable critical attention. It was the leading cause of death in female cancer patients. Ginsenoside Rg5 has been implicated in the growth of human cancer cells, but the lack of research to determine the influence mechanism has been a significant challenge for many years. In this study, ginsenoside Rg5 was used to treat HeLa cells, and RT-qPCR, Western Blotting and RNA-seq were used to analyze the differentially expressed genes in HeLa cells to study the preliminary mechanism of ginsenoside Rg5 on cervical cancer. The results of RT-qPCR and Western Blotting were in agreement with RNA-seq analysis, which showed a down-regulation of proteins in the cell cycle pathway. These results all indicate that the expression of Minichromosome Maintenance Complex Component 6 (MCM6), Cyclin A2 (CCNA2), Cyclin-dependent kinase 6 (CDK6) and Cell division cycle 6 (CDC6) in HeLa cells treated with ginsenoside Rg5 is significantly reduced, with MCM6 exhibiting the most significant inhibitory effect, making it a potential target for the treatment of cervical cancer.
宫颈癌问题已受到相当多的批判性关注。它曾是女性癌症患者的主要死因。人参皂苷Rg5与人类癌细胞的生长有关，但多年来缺乏确定其影响机制的研究一直是一个重大挑战。在本研究中，使用人参皂苷Rg5处理HeLa细胞，并采用RT-qPCR、蛋白质免疫印迹法和RNA测序分析HeLa细胞中差异表达的基因，以研究人参皂苷Rg5对宫颈癌的初步作用机制。RT-qPCR和蛋白质免疫印迹法的结果与RNA测序分析结果一致，表明细胞周期途径中的蛋白质表达下调。这些结果均表明，用人参皂苷Rg5处理的HeLa细胞中，微小染色体维持复合物组分6（MCM6）、细胞周期蛋白A2（CCNA2）、细胞周期蛋白依赖性激酶6（CDK6）和细胞分裂周期蛋白6（CDC6）的表达显著降低，其中MCM6的抑制作用最为显著，使其成为治疗宫颈癌的潜在靶点。
