Roberts L J, Aulsebrook K A, Oates J A
J Chromatogr. 1985 Feb 27;338(1):41-9. doi: 10.1016/0378-4347(85)80068-2.
Considerable inaccuracy and unreliability have recently been demonstrated to be associated with the widely used radioenzymatic methods for the determination of histamine in biological fluids. Urine appears to inhibit the methylation of histamine by histamine N-methyltransferase such that the radioenzymatic assay underestimates the concentration of histamine present in urine. Directly comparing the radioenzymatic assay with a recently developed reference method using mass spectrometry for the determination of urinary histamine, up to 34-fold differences in the levels of urinary histamine were found with the two methods.
最近已证明，广泛使用的用于测定生物体液中组胺的放射酶法存在相当大的误差和不可靠性。尿液似乎会抑制组胺N-甲基转移酶对组胺的甲基化作用，因此放射酶法会低估尿液中组胺的浓度。将放射酶法与最近开发的使用质谱法测定尿组胺的参考方法直接比较，发现两种方法测定的尿组胺水平相差高达34倍。
