van der Vusse G J, Roemen T H, Reneman R S
Biochim Biophys Acta. 1980 Feb 22;617(2):347-9. doi: 10.1016/0005-2760(80)90176-9.
The concentration and composition of fatty acids in four lipid classes in biopsies of dog left ventricular myocardium were determined, using gas-liquid chromatography. When precautions were taken to minimize lipolysis during storage of the tissue and the homogenization process, the following results were obtained: 29 +/- 10 nmol non-esterified fatty acids, 2.98 +/- 2.41 mumol triacylglycerol, 149 +/- 51 nmol cholesteryl esters and 23.76 +/- 3.38 mumol phospholipid (expressed as fatty acid moiety per gram of wet tissue). The concentration of non-esterified fatty acids was 15 to 300 times lower than reported in literature. The main constituents of the non-esterified fatty acids were palmitic, stearic and oleic acid. Triacylglycerol consisted of approximately 40% esterified oleic acid. Linoleic acid accounted for 40% of the fatty acids in the cholesteryl-esters class. More than half of the fatty acid moiety of total phospholipids was linoleic acid and arachidonic acid.
采用气液色谱法测定了犬左心室心肌活检组织中四种脂质类别的脂肪酸浓度和组成。在组织储存和匀浆过程中采取预防措施以尽量减少脂解作用后，得到以下结果：29±10 nmol非酯化脂肪酸、2.98±2.41 μmol三酰甘油、149±51 nmol胆固醇酯和23.76±3.38 μmol磷脂（以每克湿组织中的脂肪酸部分表示）。非酯化脂肪酸的浓度比文献报道的低15至300倍。非酯化脂肪酸的主要成分是棕榈酸、硬脂酸和油酸。三酰甘油约由40%的酯化油酸组成。亚油酸占胆固醇酯类别中脂肪酸的4０%。总磷脂脂肪酸部分的一半以上是亚油酸和花生四烯酸。
