Tsai M Y, Oliphant C, Josephson M W
J Chromatogr. 1985 May 31;341(1):1-10. doi: 10.1016/s0378-4347(00)84004-9.
To offer a procedure with increased resolution compared to packed-column gas chromatography, we developed a dual-capillary method of gas chromatography for diagnosing organic acidurias. We derivatized and injected organic acids repeatedly on two different bonded phase columns (DB-1, DB-1701) to establish a table of methylene units. Compounds in urine specimens were identified by their characteristic pair of methylene units. With this method, we are able to identify 120 metabolites in urine. Accordingly, the procedure provides a cost-effective alternative to routine gas chromatography-mass spectrometry.
为了提供一种与填充柱气相色谱法相比分辨率更高的方法，我们开发了一种用于诊断有机酸尿症的双毛细管气相色谱法。我们在两根不同的键合相柱（DB-1、DB-1701）上反复衍生化并注入有机酸，以建立亚甲基单元表。尿液标本中的化合物通过其特征性的亚甲基单元对来鉴定。通过这种方法，我们能够鉴定尿液中的120种代谢物。因此，该方法为常规气相色谱-质谱联用提供了一种经济高效的替代方法。
