Jellum E, Storseth P, Alexander J, Helland P, Stokke O, Teig E
J Chromatogr. 1976 Nov 3;126:487-93. doi: 10.1016/s0021-9673(01)84095-4.
Open-tubular glass capillary columns have been used in gas chromatography in combination with mass spectrometry (GC-MS) and computer methods to study human diseases. Patients with maple syrup urine disease excrete not only alpha-keto and alpha-hydroxy acids but also six other metabolites which hitherto have been overlooked. The GC-MS methods demonstrated that a group of patients suffering from hereditary progressive loss of hearing have an impaired metabolism of leucine, leading to the accumulation of 3-hydroxyisovaleric acid and 3-methylcrotonylglycine. GC using the capillary columns proved suitable for mapping of the carbohydrate profile of human seminal fluid and for the analyses of organic compounds accumulating in human adipose tissue. The high resolving power and long life of the glass capillary columns suggest that they will be valuable in the diagnosis and study of human disorders.
开管玻璃毛细管柱已用于气相色谱法，并与质谱联用（GC-MS）以及计算机方法来研究人类疾病。患有枫糖尿症的患者不仅会排出α-酮酸和α-羟基酸，还会排出其他六种迄今为止被忽视的代谢物。GC-MS方法表明，一组患有遗传性渐进性听力丧失的患者存在亮氨酸代谢受损的情况，导致3-羟基异戊酸和3-甲基巴豆酰甘氨酸的积累。使用毛细管柱的气相色谱法被证明适用于绘制人类精液的碳水化合物图谱以及分析人类脂肪组织中积累的有机化合物。玻璃毛细管柱的高分辨率和长寿命表明它们在人类疾病的诊断和研究中将具有重要价值。
