Lempiäinen M, Mäkelä A L
J Chromatogr. 1985 May 31;341(1):105-13. doi: 10.1016/s0378-4347(00)84014-1.
A method for the determination of proquazone and its m-hydroxy metabolite in serum and urine by reversed-phase high-performance liquid chromatography is described. The technique is based on a single extraction of the unchanged drug, its metabolite and an internal standard from serum or urine with chloroform. The column was packed with mu Bondapak C18 and the mobile phase was acetonitrile--water (50:50) (pH 3). The detection limits for proquazone and its metabolite were 0.02 mumol/l using 500 microliters of sample. For the determination of the total m-hydroxy metabolite only 100 microliters of sample are needed. The method described is suitable for routine clinical and pharmacokinetic studies. The clinical application of this method suggests that the pharmacokinetics of proquazone in adults and children are similar.
本文描述了一种用反相高效液相色谱法测定血清和尿液中丙酰苯哌啶及其间羟基代谢物的方法。该技术基于用氯仿从血清或尿液中单次提取未变化的药物、其代谢物和内标物。色谱柱填充μ Bondapak C18，流动相为乙腈 - 水（50:50）（pH 3）。使用500微升样品时，丙酰苯哌啶及其代谢物的检测限为0.02微摩尔/升。仅测定总间羟基代谢物时，仅需100微升样品。所描述的方法适用于常规临床和药代动力学研究。该方法的临床应用表明，丙酰苯哌啶在成人和儿童中的药代动力学相似。
