Lempiäinen M, Mäkelä A L
Int J Clin Pharmacol Res. 1987;7(4):265-71.
A method for the determination of ketoprofen in serum and urine by reversed-phase high-performance liquid chromatography is described. The technique is based on a single extraction of the drug and an internal standard from serum or urine with chloroform. The column was packed with uBondapak C18 and the mobile phase was acetonitrile-water (50:50)(pH 3). The detection limit for ketoprofen was 0.1 mumol/l using 500 microliter of sample. The method described is suitable for routine and pharmacokinetic studies. The results of its clinical application suggest that the pharmacokinetics of ketoprofen in adults and in children are similar.
本文描述了一种用反相高效液相色谱法测定血清和尿液中酮洛芬的方法。该技术基于用氯仿从血清或尿液中单次提取药物和内标物。色谱柱填充有uBondapak C18，流动相为乙腈 - 水（50:50）（pH 3）。使用500微升样品时，酮洛芬的检测限为0.1微摩尔/升。所描述的方法适用于常规和药代动力学研究。其临床应用结果表明，酮洛芬在成人和儿童中的药代动力学相似。
