Determination of ketoprofen by high-performance liquid chromatography from serum and urine: clinical application in children with juvenile rheumatoid arthritis.

A method for the determination of ketoprofen in serum and urine by reversed-phase high-performance liquid chromatography is described. The technique is based on a single extraction of the drug and an internal standard from serum or urine with chloroform. The column was packed with uBondapak C18 and the mobile phase was acetonitrile-water (50:50)(pH 3). The detection limit for ketoprofen was 0.1 mumol/l using 500 microliter of sample. The method described is suitable for routine and pharmacokinetic studies. The results of its clinical application suggest that the pharmacokinetics of ketoprofen in adults and in children are similar.