Presutti Caterina, Vreeker Edo, Sasidharan Sajitha, Ferdinando Zanetta, Stuart Marc, Juhaniewicz-Dębińska Joanna, Maglia Giovanni, Roos Wouter H, Poolman Bert
Department of Biochemistry, University of Groningen, Nijenborgh 3, Groningen 9747 AG, The Netherlands.
Chemical Biology, University of Groningen, Nijenborgh 7, Groningen 9747 AG, The Netherlands.
Biomacromolecules. 2025 May 12;26(5):2868-2881. doi: 10.1021/acs.biomac.4c01651. Epub 2025 Apr 8.
We have synthesized hybrid membranes composed of amphiphilic block copolymers, polybutadiene-poly(ethylene oxide) [PBd--PEO], with different lengths [PBd-PEO and PBd-PEO] and mixtures of phospholipids (DOPC:DOPG:DOPE 50:25:25 mol %) to combine the properties of both in terms of stability and fluidity of the membrane. The amphiphilic block copolymers increase the stability, whereas the lipids support the functionality of membrane proteins. The hybrid nature of the bilayers was studied by means of Cryo-TEM, Langmuir-Blodgett technique, atomic force microscopy (AFM), electrical measurements, and fluorescence-based stopped-flow assay to determine the permeability of the membrane for water and osmolytes. We observe that the structural, thermodynamic, and permeability properties of hybrid PBd-PEO membranes are similar to their purely lipid counterparts, with the advantage of being more stable and resisting a higher transmembrane electrical potential. Hybrid membranes with the longer polymer, PBd-PEO, display more significant structural, thermodynamic, and permeability differences and show less favorable properties than hybrid-PBd-PEO membranes.
我们合成了由两亲性嵌段共聚物聚丁二烯-聚环氧乙烷[PBd-PEO]组成的混合膜，其中PBd-PEO具有不同的长度[PBd-PEO和PBd-PEO]，并与磷脂混合物（二油酰磷脂酰胆碱：二油酰磷脂酰甘油：二油酰磷脂酰乙醇胺50:25:25摩尔%）混合，以便在膜的稳定性和流动性方面兼具二者的特性。两亲性嵌段共聚物提高了稳定性，而脂质则支持膜蛋白的功能。通过冷冻透射电子显微镜、朗缪尔-布洛杰特技术、原子力显微镜（AFM）、电学测量以及基于荧光的停流测定法研究了双层膜的混合性质，以确定膜对水和渗透溶质的渗透性。我们观察到，混合PBd-PEO膜的结构、热力学和渗透性质与其纯脂质对应物相似，其优点是更稳定且能承受更高的跨膜电势。含有较长聚合物PBd-PEO的混合膜表现出更显著的结构、热力学和渗透差异，并且与混合PBd-PEO膜相比表现出不太理想的性质。
