Li Wanjing, Xu Jinbang, Shi Dan, Wang Jingyi, Liu Tao, Yang Juan, Deng Disi
Department of Gynaecology, Fujian Maternity and Child Health Hospital College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou 350001, P.R. China.
Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610075, P.R. China.
Comb Chem High Throughput Screen. 2025 Apr 7. doi: 10.2174/0113862073368250250319001421.
Diminished ovarian reserve (DOR) is accompanied by abnormal initiation and development of primordial follicles. Reporting that curcumin can protect the ovarian reserve, we used rats as a model to explore the regulatory mechanism of curcumin on primordial follicle priming.
Curcumin restores the ovarian microenvironment of DOR model rats by AMPK/ SIRT 1 signaling pathway, thus regulating the initiation of primordial follicles.
The study used the ovaries of 3-day-old female rats, after replicating the DOR model by triptolide (TP), then used curcumin intervention for 3 days. Histomorphological analysis was counted by H & E staining; ELISA test was used to count ovarian hormone [follicle stimulating hormone (FSH) / luteinizing hormone (LH) ratio and estradiol (E2)] concentration in the culture supernatant. Spectrophotometric measurement was used to count of superoxide dismutase (SOD) and the malondialdehyde (MDA). The protein and mRNA expression of the pathway and key indicators for follicle initiation were determined by Western Blot and Q-PCR (AMPK, SIRT 1, PTEN, PGC-1 α, and AMH).
After curcumin treatment, the number of growing follicles increased (P < 0.05). FSH/LH ratio decreased but the content and expression of E2 and AMH increased (P < 0.05). The protein and mRNA expression of characteristic indicators of inhibiting primordial follicle initiation (PTEN) was decreased (P < 0.05). Oxidation-reduction-related SOD activity increased and the content of MDA decreased (P < 0.05), while the protein and mRNA expression of PGC- 1α increased (P < 0.05). The protein and mRNA expression of the pathway (AMPK, SIRT 1) were increased (P < 0.05).
Curcumin restored the ovarian local oxidant-antioxidant balance and promoted primordial follicle priming through AMPK/SIRT 1 signaling pathway in the DOR model rats.
卵巢储备功能减退（DOR）伴随着原始卵泡启动和发育异常。有报道称姜黄素可保护卵巢储备功能，我们以大鼠为模型探讨姜黄素对原始卵泡启动的调控机制。
姜黄素通过AMPK/SIRT1信号通路恢复DOR模型大鼠的卵巢微环境，从而调控原始卵泡的启动。
本研究选用3日龄雌性大鼠的卵巢，用雷公藤甲素（TP）复制DOR模型后，用姜黄素干预3天。通过苏木精-伊红（H&E）染色进行组织形态学分析；采用酶联免疫吸附测定（ELISA）检测培养上清液中卵巢激素[促卵泡生成素（FSH）/促黄体生成素（LH）比值和雌二醇（E2）]浓度。采用分光光度法测定超氧化物歧化酶（SOD）和丙二醛（MDA）含量。通过蛋白质免疫印迹法（Western Blot）和实时荧光定量聚合酶链反应（Q-PCR）检测该信号通路及卵泡启动关键指标（AMPK、SIRT1、PTEN、PGC-1α和抗苗勒管激素（AMH））的蛋白和mRNA表达。
姜黄素处理后，生长卵泡数量增加（P<0.05）。FSH/LH比值降低，但E2和AMH的含量及表达增加（P<0.05）。抑制原始卵泡启动的特征指标（PTEN）的蛋白和mRNA表达降低（P<0.05）。氧化还原相关的SOD活性增加，MDA含量降低（P<0.05），而PGC-1α的蛋白和mRNA表达增加（P<0.05）。该信号通路（AMPK、SIRT1）的蛋白和mRNA表达增加（P<0.05）。
姜黄素通过AMPK/SIRT1信号通路恢复DOR模型大鼠卵巢局部氧化-抗氧化平衡，促进原始卵泡启动。
