Zhang Bingfei, Chen Le, Mei Liping, Jia Xianbo, Chen Shiyi, Wang Jie, Yu Hengwei, Lai Songjia, Sun Wenqiang
State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Key Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Int J Mol Sci. 2025 Jul 12;26(14):6713. doi: 10.3390/ijms26146713.
Curcumin is a natural polyphenolic compound known to alleviate follicular developmental abnormalities associated with ovarian dysfunction. However, its precise molecular mechanisms remain to be fully elucidated. In this study, we systematically investigated the regulatory effects of curcumin on bovine ovarian granulosa cells through integrated transcriptomic and metabolomic analyses. A total of 503 and 200 significantly altered metabolites were identified in the positive and negative ion modes, respectively, with enrichment in key pathways such as glutathione metabolism, fatty acid biosynthesis, and the phosphatidylinositol signaling pathway. Transcriptomic profiling revealed 1168 differentially expressed genes (582 upregulated and 586 downregulated) which were significantly enriched in pathways related to glutathione metabolism and cellular senescence. Joint multi-omics analysis further demonstrated that curcumin significantly influenced pathways related to glutathione metabolism, cysteine, and methionine metabolism, as well as multiple forms of programmed cell death, including apoptosis, necroptosis, and ferroptosis. Collectively, these findings suggest that curcumin may enhance the antioxidant capacity and survival of granulosa cells by maintaining redox homeostasis and modulating cell fate. This work provides new insights into the potential cellular mechanisms underlying the protective effects of curcumin on granulosa cell function.
姜黄素是一种天然多酚化合物，已知可缓解与卵巢功能障碍相关的卵泡发育异常。然而，其确切的分子机制仍有待充分阐明。在本研究中，我们通过整合转录组学和代谢组学分析，系统地研究了姜黄素对牛卵巢颗粒细胞的调节作用。在正离子和负离子模式下，分别鉴定出503种和200种显著改变的代谢物，这些代谢物在谷胱甘肽代谢、脂肪酸生物合成和磷脂酰肌醇信号通路等关键途径中富集。转录组分析揭示了1168个差异表达基因（582个上调和586个下调），这些基因在与谷胱甘肽代谢和细胞衰老相关的途径中显著富集。联合多组学分析进一步表明，姜黄素显著影响与谷胱甘肽代谢、半胱氨酸和蛋氨酸代谢相关的途径，以及多种形式的程序性细胞死亡，包括凋亡、坏死性凋亡和铁死亡。总的来说，这些发现表明姜黄素可能通过维持氧化还原稳态和调节细胞命运来增强颗粒细胞的抗氧化能力和存活率。这项工作为姜黄素对颗粒细胞功能保护作用的潜在细胞机制提供了新的见解。
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