Walker A G, Chapman J A, Rumsby M G
J Neuroimmunol. 1985 Aug;9(3-4):159-77. doi: 10.1016/s0165-5728(85)80016-3.
Subcultures have been established from primary rat brain cell cultures and have been characterised with a range of cell-specific immunocytochemical markers. The subcultures are mainly composed of fibrous astrocytes, oligodendrocytes and neurones. The cells do not divide to any great extent giving a system where it is possible to follow culture development at the cellular level for a number of weeks. During this time oligodendrocytes colonise subpopulations of neurones, differentiate further showing the presence of myelin basic protein and elaborate myelin-like membrane; the fibrous astrocytes remain scattered uniformly throughout the cultures. Radially oriented processes emerge from the oligodendrocyte-neurone aggregates which subsequently coalesce to form fascicles that link the clusters of cells together. These fascicles react with antibodies for both neurofilament protein and myelin basic protein. The subcultures provide a straightforward system that is composed of cells derived entirely from the CNS, is free from mitotic inhibitors and yet retains a sufficiently low cell density to allow immunocytochemical identification of the cell types present. The subcultures should be useful for the study of trophic interactions between oligodendrocytes and neurones as well as the early events associated with myelinogenesis.
已从原代大鼠脑细胞培养物中建立了亚培养物，并用一系列细胞特异性免疫细胞化学标记物对其进行了表征。亚培养物主要由纤维状星形胶质细胞、少突胶质细胞和神经元组成。这些细胞在很大程度上不会分裂，从而形成了一个系统，在这个系统中可以在细胞水平上追踪数周的培养物发育情况。在此期间，少突胶质细胞定殖于神经元亚群，进一步分化，显示出髓鞘碱性蛋白的存在，并形成类似髓鞘的膜；纤维状星形胶质细胞则均匀地散布在整个培养物中。从少突胶质细胞 - 神经元聚集体中出现放射状排列的突起，这些突起随后合并形成束，将细胞簇连接在一起。这些束对神经丝蛋白和髓鞘碱性蛋白的抗体均有反应。亚培养物提供了一个简单的系统，该系统由完全源自中枢神经系统的细胞组成，不含丝裂抑制剂，且细胞密度足够低，能够对存在的细胞类型进行免疫细胞化学鉴定。这些亚培养物对于研究少突胶质细胞与神经元之间的营养相互作用以及与髓鞘形成相关的早期事件应该是有用的。
