兔子对神经末梢神经节苷脂的抗体反应。通过脂质体裂解和裂解抑制试验分析抗体特异性。

Antibody response of rabbits to nerve ending gangliosides. Analysis of antibody specificity by liposome lysis and lysis inhibition assays.

作者信息

Uemura K, Kitazawa N, Taketomi T

出版信息

J Neuroimmunol. 1985 Sep;9(5):269-80. doi: 10.1016/s0165-5728(85)80025-4.

PMID:4019749

Abstract

Rabbits were immunized with nerve ending fraction prepared from guinea pig brain. Serum antibodies to total gangliosides were followed by enzyme-linked immunosorbent assay; their titers were highest at 2 to 3 weeks after immunization and some rabbits showed a response to reinjections. Specific reactivities of the antibodies against each molecular species of gangliosides were analyzed by liposome lysis assay and liposome lysis inhibition assay. Antibody responses were detected against GM1, GD1b, GM3 and GM2, but not against GD1a and GT1b. Natural and immune antibodies to the asialo glycolipids and to galactosylceramide were also observed.

摘要

用从豚鼠脑制备的神经末梢部分对兔子进行免疫。通过酶联免疫吸附测定法追踪针对总神经节苷脂的血清抗体；其滴度在免疫后2至3周时最高，并且一些兔子对再次注射有反应。通过脂质体裂解测定法和脂质体裂解抑制测定法分析抗体针对每种神经节苷脂分子种类的特异性反应性。检测到针对GM1、GD1b、GM3和GM2的抗体反应，但未检测到针对GD1a和GT1b的反应。还观察到了针对去唾液酸糖脂和半乳糖神经酰胺的天然抗体和免疫抗体。