Zhao Hehe, Jiang Jun, Chai Chengliang, Pan Xiaohong, Lyu Fan, Xing Hui, Feng Yi, Cheng Wei, Li Guixia, Mei Jianhua, Zhong Ping, Guo Zhihong, Zhou Xin, Fan Qin, Zhang Jiafeng
National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.
Department of HIV/AIDS and STDs Control and Prevention, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang, 310051, China.
Infection. 2025 Apr 8. doi: 10.1007/s15010-025-02530-y.
To understand the geographic origins, transmission hotspots, and drug resistance mutations (DRMs) of HIV-1 CRF08_BC in Zhejiang Province, China.
This study analyzed HIV-1 CRF08_BC pol sequences collected between 2020 and 2023. Bayesian inference was employed to investigate temporal epidemic trends, while HIV-TRACE and MCODE were used to identify transmission clusters (TCs), key hotspots and super-spreaders. DRMs associated with CRF08_BC were also characterized. Additionally, demographic data were integrated with these findings, allowing for a description of the transmission dynamics.
This study revealed that CRF08_BC strains in Zhejiang likely originated from Guangxi, with significant transmission among individuals aged 50 and older, particularly those with low educational levels. Molecular transmission analysis showed that 58.9% of CRF08_BC sequences were in TCs, with geographic concentrations in Taizhou (TZ) and Lishui (LS). 14 large clusters maintained effective reproductive numbers (Re) above 1, representing considerable epidemic growth. Hangzhou (HZ) emerged as a key transmission hub, with 10 TCs showing active transmission. LS established strong epidemiological links with HZ, Ningbo (NB), Taizhou (TZ), and Wenzhou (WZ), creating a pattern of viral spread radiating from LS to surrounding areas. DRMs were identified in 76 cases (6.0%), with NNRTI and NRTI mutations exhibiting distinct geographic clustering.
The CRF08_BC strains in Zhejiang likely originated from Guangxi and are mainly found in individuals aged 50 and older with low education. The current epidemic hotspots are in TZ and LS, where NNRTI and NRTI mutations are clustered, significantly impacting treatment efficacy.
了解中国浙江省HIV-1 CRF08_BC的地理起源、传播热点和耐药突变（DRMs）。
本研究分析了2020年至2023年期间收集的HIV-1 CRF08_BC pol序列。采用贝叶斯推断研究时间流行趋势，同时使用HIV-TRACE和MCODE识别传播簇（TCs）、关键热点和超级传播者。还对与CRF08_BC相关联的DRMs进行了特征描述。此外，将人口统计学数据与这些研究结果相结合，以便描述传播动态。
本研究表明，浙江的CRF08_BC毒株可能起源于广西，在50岁及以上人群中传播显著，尤其是教育程度较低的人群。分子传播分析显示，58.9%的CRF08_BC序列属于传播簇，在台州（TZ）和丽水（LS）存在地理聚集现象。14个大簇的有效繁殖数（Re）维持在1以上，代表着可观的疫情增长。杭州（HZ）成为关键传播枢纽，有10个传播簇呈现活跃传播。丽水与杭州、宁波（NB）、台州和温州（WZ）建立了紧密的流行病学联系，形成了从丽水向周边地区辐射的病毒传播模式。在76例（6.0%）病例中鉴定出耐药突变，非核苷类逆转录酶抑制剂（NNRTI）和核苷类逆转录酶抑制剂（NRTI）突变呈现出明显的地理聚集现象。
浙江的CRF08_BC毒株可能起源于广西，主要见于50岁及以上且教育程度低的人群。当前的疫情热点在台州和丽水，其中NNRTI和NRTI突变聚集，对治疗效果有显著影响。
