Ahmadi Masoumeh, Zare Afshin, Jafarzadeh Shirazi Mohammad Reza, Askari Samaneh, Khoradmehr Arezoo, Mussin Nadiar M, Kaliyev Asset A, Mohammad Rezazadeh Farzad, Saedi Saman, Dadpasand Taromsari Mohammad, Boostani Alidad, Tanideh Nader, Zhilisbayeva Kulyash R, Safarzoda Sharofiddin Ramazon, Tamadon Amin
Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran.
PerciaVista R and D Co., Shiraz, Iran.
Int J Fertil Steril. 2025 Mar 11;19(2):200-218. doi: 10.22074/ijfs.2024.2020015.1608.
traditionally employed or treating hormonal problems in women. Therefore, this study aimed to assess the effect of hydroalcoholic extract of on letrozole-induced polycystic ovary syndrome (PCOS) in adult rats.
In this experimental study, hydroalcoholic extract was prepared from the whole plant except the root. Then, 16 female Sprague-Dawley rats were divided into four groups. In the control group, the PCOS model was not induced and no treatment was performed. The PCOS-induced groups received 1 mg/kg of body-weight letrozole daily by gavage for 21 days. In the PCOS-induced groups two groups were orally treated with 250 mg/kg body weight daily metformin or 500 mg/kg body weight daily extract, 28 days after PCOS induction. Hormonal, histopathologic, and histomorphometric analyses were performed. Molecular docking also done to evaluate effect of extract on receptors involved in the pathophysiology of PCOS.
had a remarkable positive effect on estrous cycles and also led to a significant reduction in the weight of PCOS rats. Moreover, extract mitigates PCOS-induced hormonal imbalances including a significant decrease in testosterone and estrogen levels, as well as increased progesterone levels. Ovarian and uterine structures were improved including reducing theca layer thickness, enhancing antral follicular areas, and a significant decrease in the luminal area of the perimetrial layer, endometrial glands, and the total uterine area when compared to the PCOS group. Besides, molecular docking analysis showed that Ar-tumerone, Tumerone, Tricyclo[6.3.0.0(1,5)] undec-2-en-4-one, 2,3,5,9- tetramethyl, Curlone, and 3-Tert-butyl-4-hydroxyanisole showed the most binding affinity to the receptors that play crucial role in the pathophysiology of PCOS.
extract could display positive effects on both the ovary and uterus of letrozole-induced PCOS rats. Therefore, had therapeutic effects on the ovary and uterus of PCOS-induced rat models.
传统上用于治疗女性激素问题。因此，本研究旨在评估[提取物名称]水醇提取物对来曲唑诱导的成年大鼠多囊卵巢综合征（PCOS）的影响。
在本实验研究中，从除根以外的全株植物中制备水醇提取物。然后，将16只雌性Sprague-Dawley大鼠分为四组。对照组未诱导PCOS模型，也未进行治疗。PCOS诱导组每天通过灌胃给予1mg/kg体重的来曲唑，持续21天。在PCOS诱导组中，两组在PCOS诱导28天后分别口服250mg/kg体重的二甲双胍或500mg/kg体重的[提取物名称]提取物。进行了激素、组织病理学和组织形态计量学分析。还进行了分子对接以评估[提取物名称]提取物对PCOS病理生理学中涉及的受体的影响。
[提取物名称]对发情周期有显著的积极影响，还导致PCOS大鼠体重显著减轻。此外，[提取物名称]提取物减轻了PCOS诱导的激素失衡，包括睾酮和雌激素水平显著降低，以及孕酮水平升高。与PCOS组相比，卵巢和子宫结构得到改善，包括卵泡膜层厚度降低、窦状卵泡面积增加，以及子宫肌层、子宫内膜腺体的管腔面积和子宫总面积显著减小。此外，分子对接分析表明，Ar-莪术酮、莪术酮、三环[6.3.0.0(1,5)]十一碳-2-烯-4-酮、2,3,5,9-四甲基、卷曲酮和3-叔丁基-4-羟基茴香醚对在PCOS病理生理学中起关键作用的受体显示出最强的结合亲和力。
[提取物名称]提取物对来曲唑诱导的PCOS大鼠的卵巢和子宫均显示出积极作用。因此，[提取物名称]对PCOS诱导的大鼠模型的卵巢和子宫具有治疗作用。
