Malpani Arati, Mahurkar Nitin, Aswar Urmila
Department of Pharmacology, H.K.E.'s MTR Institute of Pharmaceutical Sciences, Kalaburagi 585105, India.
Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Pune 411041, India.
Chin Herb Med. 2020 Jun 10;12(3):281-288. doi: 10.1016/j.chmed.2020.06.001. eCollection 2020 Jul.
The objective of the present study was to evaluate the antifertility activity of ether (ErCD), chloroform (CeCD) and ethyl alcohol (EyCD) extracts of the whole plant of in female Wistar albino rats.
Acute oral toxicity and an antifertility study were performed in female Wistar rats with two dose levels (200 and 400 mg/kg, orally) of EyCD. The estrogenic and progestogenic effects of EyCD were further observed by administering it to immature Wistar rats by investigations of vaginal cornification, hormonal level, uterus weight, biochemical parameters, histopathology of the uterus and deciduoma formation, respectively. Isolation of EyCD was carried out by Flash Chromatography and isolated fraction was estimated by HPLC.
No toxicity with any of the extract was found up to the dose of 2000 mg/kg body weight. EyCD treated rats exhibited maximum reduction in pregnancy (83.33%). Estimation of EyCD on vaginal cornification, estrogen-induced uterotrophic assay and deciduoma model demonstrated vaginal cornification, significant ( < 0.01) increase in uterine weight and uterine proliferation in histopathology and reduced deciduoma formation respectively. Hormonal and biochemical parameters confirmed the above findings indicating estrogenic potential and antiprogestogenic potential of EyCD that might be attributed to the presence of phytoestrogen (apigenin) in EyCD.
The results suggested that extracts of possess significant antifertility activity, which is consistent with the literature reported in folk medicine of this plant in fertility regulation.
本研究的目的是评估[植物名称]全株的乙醚提取物（ErCD）、氯仿提取物（CeCD）和乙醇提取物（EyCD）对雌性Wistar白化大鼠的抗生育活性。
对雌性Wistar大鼠进行急性经口毒性试验和抗生育研究，给予两个剂量水平（200和400mg/kg，经口）的EyCD。通过分别观察阴道角化、激素水平、子宫重量、生化参数、子宫组织病理学和蜕膜瘤形成，进一步观察EyCD对未成熟Wistar大鼠的雌激素和孕激素作用。采用快速色谱法分离EyCD，并通过高效液相色谱法估算分离出的馏分。
在高达2000mg/kg体重的剂量下，未发现任何提取物有毒性。EyCD处理的大鼠妊娠率降低最多（83.33%）。对EyCD进行阴道角化、雌激素诱导的子宫增重试验和蜕膜瘤模型评估，结果显示阴道角化、子宫重量显著增加（P<0.01）、组织病理学上子宫增殖以及蜕膜瘤形成减少。激素和生化参数证实了上述结果，表明EyCD具有雌激素潜力和抗孕激素潜力，这可能归因于EyCD中存在植物雌激素（芹菜素）。
结果表明，[植物名称]的提取物具有显著的抗生育活性，这与该植物民间医学中关于生育调节的文献报道一致。
