Tatebe Yuka, Yamamoto Yuri, Isono Naoto
1 Faculty of Bioresources, Mie University.
2 Graduate School of Bioresources, Mie University.
J Appl Glycosci (1999). 2025 Feb 20;72(1):7201201. doi: 10.5458/jag.7201201. eCollection 2025.
In this study, we developed a method to synthesize sophorose using three enzymes-sucrose phosphorylase from , 1,2-β-oligoglucan phosphorylase from , and exo β-1,2-glucooligosaccharide sophorohydrolase from -in a one-pot reaction, employing inexpensive starting materials. After optimization, a reaction was carried out using 5 mM glucose, 250 mM sucrose, 10 mM inorganic phosphate, and enzyme concentrations of 5 µg/mL sucrose phosphorylase, 20 µg/mL 1,2-β-oligoglucan phosphorylase, and 50 µg/mL exo β-1,2-glucooligosaccharide sophorohydrolase at 30 °C for 48 h, yielding 108 mM sophorose. Following yeast treatment, sophorose was purified by size-exclusion chromatography with a final yield of 45 % based on the amount of sucrose used as the donor substrate.
在本研究中，我们开发了一种方法，使用三种酶（来自[具体来源1]的蔗糖磷酸化酶、来自[具体来源2]的1,2-β-寡聚糖磷酸化酶和来自[具体来源3]的外切β-1,2-葡萄糖寡糖槐糖水解酶）在一锅反应中合成槐糖，采用廉价的起始原料。优化后，在30℃下使用5 mM葡萄糖、250 mM蔗糖、10 mM无机磷酸盐以及5 µg/mL蔗糖磷酸化酶、20 µg/mL 1,2-β-寡聚糖磷酸化酶和50 µg/mL外切β-1,2-葡萄糖寡糖槐糖水解酶的酶浓度进行反应48小时，得到108 mM槐糖。经过酵母处理后，通过尺寸排阻色谱法纯化槐糖，基于用作供体底物的蔗糖量，最终产率为45%。
