Graduate School of Bioresources, Mie University, Tsu, Japan.
Faculty of Bioresources, Mie University, Tsu, Japan.
Biosci Biotechnol Biochem. 2023 Sep 21;87(10):1249-1253. doi: 10.1093/bbb/zbad099.
YcjT is a kojibiose phosphorylase found in Escherichia coli. We found that sucrose was a good acceptor of YcjT in reverse phosphorolysis using β-d-glucose 1-phosphate as a donor. The product was identified as β-d-fructofuranosyl α-d-glucopyranosyl-(1→2)-α-d-glucopyranoside. This sugar was also synthesized from sucrose and maltose using YcjT and maltose phosphorylase and promoted the growth of the probiotic Bifidobacterium breve.
YcjT 是大肠杆菌中的一种昆布二糖磷酸化酶。我们发现，在以β-d-葡萄糖 1-磷酸作为供体的反向磷酸解中，蔗糖是 YcjT 的良好受体。产物被鉴定为β-d-呋喃果糖基α-d-吡喃葡萄糖基-(1→2)-α-d-吡喃葡萄糖苷。该糖也可使用 YcjT 和麦芽糖磷酸化酶从蔗糖和麦芽糖合成，并促进益生菌短双歧杆菌的生长。
