Maquart F X, Bellon G, Cornillet-Stoupy J, Randoux A, Triller R, Kalis B, Borel J P
J Invest Dermatol. 1985 Aug;85(2):156-60. doi: 10.1111/1523-1747.ep12276586.
It was shown in a previous paper that a connective tissue glycoprotein (CTGP) extracted from normal rabbit dermis was able to inhibit total protein and collagen syntheses by normal dermis fibroblast cultures. In the present study, the effects of CTGP on scleroderma fibroblasts were investigated. [14C]Proline incorporation into total proteins of the supernatant was not significantly different from that found in controls. By contrast, the amount of collagen, expressed as percentage of total secreted protein, was far higher in scleroderma cultures than in normal ones (14.4% +/- 6.0% vs 4.6% +/- 0.9%). Addition of CTGP to the medium induced a concentration-dependent inhibition of [14C]proline incorporation into proteins from both control and scleroderma cells. In control cultures, no significant decrease of the percentage of collagen was observed, but over 60 micrograms/ml, both cytotoxic effects and inhibition of protein synthesis occurred. In scleroderma cultures, the inhibition was twice as effective on collagen as on noncollagen protein synthesis. The inhibition of collagen secretion was not related either to changes in collagen hydroxylation or to the intracellular catabolism of newly synthesized procollagen.
先前的一篇论文表明，从正常兔真皮中提取的一种结缔组织糖蛋白（CTGP）能够抑制正常真皮成纤维细胞培养物中的总蛋白和胶原蛋白合成。在本研究中，研究了CTGP对硬皮病成纤维细胞的影响。上清液总蛋白中[¹⁴C]脯氨酸的掺入量与对照组相比无显著差异。相比之下，以分泌总蛋白的百分比表示的胶原蛋白量，在硬皮病培养物中比正常培养物中高得多（14.4%±6.0%对4.6%±0.9%）。向培养基中添加CTGP会导致[¹⁴C]脯氨酸掺入对照细胞和硬皮病细胞蛋白质的过程受到浓度依赖性抑制。在对照培养物中，未观察到胶原蛋白百分比的显著降低，但超过60微克/毫升时，会出现细胞毒性作用和蛋白质合成抑制。在硬皮病培养物中，对胶原蛋白的抑制作用是非胶原蛋白合成的两倍。胶原蛋白分泌的抑制既与胶原蛋白羟化的变化无关，也与新合成的前胶原的细胞内分解代谢无关。
