Wahyunengtiyas Marita, Amalia Amirul, Widjiati Widjiati, Warsito Sunaryo Hadi, Rachmawati Kadek, Hamid Iwan Sahrial, Plumeriastuti Hani, Hendrawan Viski Fitri
Students of Magister of Biology Reproduction, Faculty of Veterinary Medicine, University of Airlangga, Surabaya, Indonesia.
Students of Doctoral, Faculty of Medicine, University of Airlangga, Surabaya, Indonesia.
Open Vet J. 2025 Feb;15(2):709-713. doi: 10.5455/OVJ.2025.v15.i2.19. Epub 2025 Feb 28.
Cigarette smoke is known to have harmful effects on the brain, particularly on glial cells, which play a crucial role in maintaining the health and functionality of the central nervous system. (black cumin) has been reported to have neuroprotective properties, but its effects on glial cells in the cerebellum and cerebrum when exposed to cigarette smoke are not well understood.
This study aimed to determine the benefits of on the number of glial cells in the cerebellum and cerebrum of white rats () exposed to cigarette smoke.
Thirty white rats weighing approximately 105 g were divided into five groups: K (control group, no exposure to cigarette smoke and without given ), K+ (exposed to cigarette smoke, 2 cigarettes/day, and 0.5% CMC), and three treatment groups (P1, P2, and P3) exposed to cigarette smoke, 2 cigarettes/day, with dosages of 300, 600, and 1200 mg/kg BW, respectively. The number of glial cells in the cerebellum and cerebrum was examined under a microscope at 400x magnification. Data were analyzed using a one-way ANOVA test, followed by the Duncan test.
Significant difference ( < 0.05) in the number of glial cells in both the cerebellum and cerebrum between the control and treatment groups.
The administration of , particularly at a dosage of 1,200 mg/kg BW/day, significantly increased and maintained the number of glial cells in the cerebellum and cerebrum of rats exposed to cigarette smoke.
众所周知，香烟烟雾对大脑有有害影响，尤其是对神经胶质细胞，而神经胶质细胞在维持中枢神经系统的健康和功能方面起着至关重要的作用。据报道，黑种草籽具有神经保护特性，但在暴露于香烟烟雾时，其对小脑和大脑中神经胶质细胞的影响尚不清楚。
本研究旨在确定黑种草籽对暴露于香烟烟雾的白色大鼠（）小脑和大脑中神经胶质细胞数量的益处。
将30只体重约105克的白色大鼠分为五组：K组（对照组，未暴露于香烟烟雾且未给予黑种草籽）、K+组（暴露于香烟烟雾，每天2支香烟，给予0.5%羧甲基纤维素），以及三个暴露于香烟烟雾的治疗组（P1、P2和P3），每天2支香烟，黑种草籽剂量分别为300、600和1200毫克/千克体重。在400倍放大倍数下，在显微镜下检查小脑和大脑中神经胶质细胞的数量。使用单因素方差分析检验分析数据，随后进行邓肯检验。
对照组和治疗组之间小脑和大脑中神经胶质细胞数量存在显著差异（<0.05）。
给予黑种草籽，特别是剂量为1200毫克/千克体重/天，可显著增加并维持暴露于香烟烟雾的大鼠小脑和大脑中神经胶质细胞的数量。
