Glembová Veronika, Tóthová Csilla, Oršuľaková Veronika, Nagy Oskar
Clinic of Ruminants, University of Veterinary Medicine and Pharmacy in Kośice, Kośice, Slovak Republic.
Open Vet J. 2025 Feb;15(2):893-900. doi: 10.5455/OVJ.2025.v15.i2.38. Epub 2025 Feb 28.
In high-yielding dairy ewes, metabolic and reproductive processes occurring around the time of parturition and during lactation initiation are accompanied by several changes in blood biochemical parameters, including those related to protein metabolism. However, little is known about the distribution of serum proteins throughout the peripartum period in ewes without higher economic productivity.
This study aimed to evaluate changes in serum protein profile throughout the peripartal period in ewes with milk production only for the nutritional needs of lambs.
Twelve clinically healthy ewes of Merino and Cigája crossbreeds at the late stage of pregnancy were included in the study. They were evaluated from 1 month before the expected lambing to 8 weeks after lambing. Blood samples were collected 4 and 2 weeks before the expected parturition and then 2, 4, 6, and 8 weeks . The serum samples were analyzed for the concentrations of total serum proteins and the main protein fractions separated by agarose gel electrophoresis.
Six protein fractions were identified on the serum protein electrophoretogram of the ewes, including albumin, α-, α-, β-, γ, and γ-globulins. The concentrations of total serum proteins did not significantly vary throughout the evaluated peripartal period but decreased only slightly around the time of parturition. Significant changes were observed in the relative values and distribution of α-and γ-globulin fractions ( < 0.05 and < 0.01). The absolute albumin concentrations were lowest 4 weeks before lambing and highest in the later postpartal period ( < 0.001). The γ-globulin values showed a significant gradual decrease until 4 weeks after lambing ( < 0.05 and < 0.01). Significant changes were observed in the concentrations of γ-globulins ( < 0.01), with the highest values in the later postpartal period similar to γ-globulins.
The results suggest that changes in the concentration and distribution of serum protein fractions around the time of parturition also occur in ewes with low milk production and may help to better understand the physiological adaptations, that accompany the female's organism during the transitional period.
在高产奶山羊中，分娩前后及泌乳初期发生的代谢和生殖过程伴随着血液生化参数的若干变化，包括与蛋白质代谢相关的参数。然而，对于经济生产力不高的山羊在围产期血清蛋白的分布情况知之甚少。
本研究旨在评估仅为满足羔羊营养需求而产奶的山羊在围产期血清蛋白谱的变化。
本研究纳入了12只处于妊娠后期的临床健康的美利奴和西加亚杂交母羊。从预计产羔前1个月至产羔后8周对它们进行评估。在预计分娩前4周和2周，然后在产后2、4、6和8周采集血样。对血清样本进行总血清蛋白浓度分析以及通过琼脂糖凝胶电泳分离的主要蛋白组分分析。
在母羊血清蛋白电泳图上鉴定出六种蛋白组分，包括白蛋白、α1-、α2-、β-、γ1和γ2球蛋白。在评估的围产期内，总血清蛋白浓度没有显著变化，但在分娩前后仅略有下降。观察到α1-和γ球蛋白组分的相对值和分布有显著变化（P<0.05和P<0.01）。绝对白蛋白浓度在产羔前4周最低，在产后后期最高（P<0.001）。γ1球蛋白值在产羔后4周前呈显著逐渐下降趋势（P<0.05和P<0.01）。观察到γ2球蛋白浓度有显著变化（P<0.01），产后后期的最高值与γ1球蛋白相似。
结果表明，产奶量低的母羊在分娩前后血清蛋白组分的浓度和分布也会发生变化，这可能有助于更好地理解雌性机体在过渡期所伴随的生理适应情况。
