Department of Experimental Sciences and Applied Biotechnology. Faculty of Veterinary Medicine. Polo Universitario Annunziata, University of Messina, Messina, Italy.
J Dairy Res. 2011 Nov;78(4):421-5. doi: 10.1017/S0022029911000562. Epub 2011 Aug 16.
In dairy cows the period of transition from late gestation to early lactation is recognized as inducing considerable metabolic adaptation. The aim of this study was to analyse modifications in serum protein values occurring during the dry and the transition period and during lactation in a group of five Holstein cows of high average milk production. For all subjects, selected on the basis of their pregnancy status, blood samples were collected at different physiological phases: dry period (-60, -30 d to calving), transition period (almost 7 d to calving, 7 d after calving), and lactation (weeks 2, 5 and 15 after calving), for a total of eight blood samples for each cow. On each blood sample total proteins and electrophoresis analysis were performed. On the data obtained, normally distributed (P<0·05, Kolmogorov-Smirnov's test), one-way Repeated Measure Analysis of Variance (ANOVA), was applied to evaluate the influence of different stages of gestation and lactation on the considered parameters. Results showed a significant effect on total proteins, α1-globulins, β-globulins, γ-globulins and albumin/globulin ratio. Most of the detected modifications were related to the transition from gestation to lactation, indicating that it is a period of great metabolic stress for cows. On the basis of the obtained results we can affirm that the pattern of serum protein fraction rn could give information about dehydration, plasma volume expansion and hepatic function occurring during the peripartum period in dairy cows.
在奶牛中，从妊娠后期到泌乳早期的过渡期被认为会引起相当大的代谢适应。本研究的目的是分析高产荷斯坦奶牛在干奶期、过渡期和泌乳期血清蛋白值的变化。选择处于妊娠状态的奶牛，在不同的生理阶段采集血液样本：干奶期（产前-60 天至-30 天）、过渡期（产前近 7 天，产后 7 天）和泌乳期（产后 2、5 和 15 周），每个牛共采集 8 个血样。对每个血样进行总蛋白和电泳分析。对获得的数据进行正态分布检验（P<0.05，Kolmogorov-Smirnov 检验），应用单向重复测量方差分析（ANOVA）评估不同妊娠和泌乳阶段对所考虑参数的影响。结果表明，总蛋白、α1-球蛋白、β-球蛋白、γ-球蛋白和白蛋白/球蛋白比值有显著影响。大多数检测到的变化与从妊娠到泌乳的过渡有关，这表明过渡期是奶牛代谢应激的一个重要时期。根据获得的结果，我们可以肯定血清蛋白组分模式可以提供有关围产期奶牛脱水、血浆容量扩张和肝功能的信息。
