• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

血浆来源的外泌体i-tRF-LeuCAA作为胶质瘤诊断的生物标志物及通过TPM4调控促进上皮-间质转化的因子

Plasma-Derived Exosomal i-tRF-LeuCAA as Biomarker for Glioma Diagnosis and Promoter of Epithelial-Mesenchymal Transition via TPM4 Regulation.

作者信息

Liu Hongyu, Hu Wentao, Zhang Lijun, Li Ze, Liu Jialin, Chen Ling

机构信息

Medical School of Chinese PLA, Beijing, China.

Department of Neurosurgery, Hainan Hospital of Chinese PLA General Hospital, Sanya, Hainan, China.

出版信息

CNS Neurosci Ther. 2025 Apr;31(4):e70356. doi: 10.1111/cns.70356.

DOI:10.1111/cns.70356
PMID:40202170
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11979793/
Abstract

AIMS

This study aimed to discover plasma-derived exosomal tsRNAs that serve as novel diagnostic biomarkers for glioma and to investigate the mechanism by which tsRNAs regulate glioma development.

METHODS

Differentially expressed tsRNAs in the plasma exosomes of glioma patients were identified using small RNA array sequencing. Bioinformatics analyses were used to predict the biological function of tsRNAs. The changes in the phenotypes of glioma cells treated with a tsRNA mimic and inhibitor were detected. The diagnostic and prognostic characteristics of potential target genes and their related functions in gliomas were further analyzed. The cell and animal experiments were used to analyze the molecular mechanisms.

RESULTS

Among the 453 differentially expressed tsRNAs identified in the plasma-derived exosomes of glioma patients using small RNA sequencing, i-tRF-LeuCAA was associated with the prognosis and molecular diagnostic characteristics of glioma patients and promoted the migration, invasion, and proliferation of glioma cells and inhibited their apoptosis. In addition, TPM4 is a potential target of i-tRF-LeuCAA and is related to epithelial-mesenchymal transition in gliomas.

CONCLUSIONS

i-tRF-LeuCAA could be served as a non-invasive biomarker in the diagnosis and prognosis of glioma. i-tRF-LeuCAA may indirectly regulate TPM4 expression and influence epithelial-mesenchymal transition, which may promote glioma progression.

摘要

目的

本研究旨在发现可作为胶质瘤新型诊断生物标志物的血浆来源外泌体tsRNAs，并探究tsRNAs调控胶质瘤发展的机制。

方法

使用小RNA阵列测序鉴定胶质瘤患者血浆外泌体中差异表达的tsRNAs。利用生物信息学分析预测tsRNAs的生物学功能。检测用tsRNA模拟物和抑制剂处理的胶质瘤细胞表型变化。进一步分析潜在靶基因在胶质瘤中的诊断和预后特征及其相关功能。通过细胞和动物实验分析分子机制。

结果

使用小RNA测序在胶质瘤患者血浆来源外泌体中鉴定出的453个差异表达的tsRNAs中，i-tRF-LeuCAA与胶质瘤患者的预后和分子诊断特征相关，并促进胶质瘤细胞的迁移、侵袭和增殖，抑制其凋亡。此外，TPM4是i-tRF-LeuCAA的潜在靶标，且与胶质瘤中的上皮-间质转化有关。

结论

i-tRF-LeuCAA可作为胶质瘤诊断和预后的非侵入性生物标志物。i-tRF-LeuCAA可能间接调节TPM4表达并影响上皮-间质转化，从而促进胶质瘤进展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66ba/11979793/41c8a5694bc7/CNS-31-e70356-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66ba/11979793/8f71d06e29f4/CNS-31-e70356-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66ba/11979793/078b1ad22348/CNS-31-e70356-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66ba/11979793/0e06faf725c2/CNS-31-e70356-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66ba/11979793/a0235848b3ce/CNS-31-e70356-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66ba/11979793/efb76e47bad4/CNS-31-e70356-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66ba/11979793/9803a4e11e74/CNS-31-e70356-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66ba/11979793/ac508730e970/CNS-31-e70356-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66ba/11979793/1e00743d54fd/CNS-31-e70356-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66ba/11979793/570cafeed0b3/CNS-31-e70356-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66ba/11979793/41c8a5694bc7/CNS-31-e70356-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66ba/11979793/8f71d06e29f4/CNS-31-e70356-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66ba/11979793/078b1ad22348/CNS-31-e70356-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66ba/11979793/0e06faf725c2/CNS-31-e70356-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66ba/11979793/a0235848b3ce/CNS-31-e70356-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66ba/11979793/efb76e47bad4/CNS-31-e70356-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66ba/11979793/9803a4e11e74/CNS-31-e70356-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66ba/11979793/ac508730e970/CNS-31-e70356-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66ba/11979793/1e00743d54fd/CNS-31-e70356-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66ba/11979793/570cafeed0b3/CNS-31-e70356-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66ba/11979793/41c8a5694bc7/CNS-31-e70356-g002.jpg

相似文献

1
Plasma-Derived Exosomal i-tRF-LeuCAA as Biomarker for Glioma Diagnosis and Promoter of Epithelial-Mesenchymal Transition via TPM4 Regulation.血浆来源的外泌体i-tRF-LeuCAA作为胶质瘤诊断的生物标志物及通过TPM4调控促进上皮-间质转化的因子
CNS Neurosci Ther. 2025 Apr;31(4):e70356. doi: 10.1111/cns.70356.
2
Transfer RNA-derived small RNAs (tsRNAs) sequencing revealed a differential expression landscape of tsRNAs between glioblastoma and low-grade glioma.转运RNA衍生的小RNA（tsRNAs）测序揭示了胶质母细胞瘤和低级别胶质瘤之间tsRNAs的差异表达图谱。
Gene. 2023 Mar 1;855:147114. doi: 10.1016/j.gene.2022.147114. Epub 2022 Dec 13.
3
Mesenchymal stem cell-derived exosomal microRNA-133b suppresses glioma progression via Wnt/β-catenin signaling pathway by targeting EZH2.间质干细胞衍生的外泌体 microRNA-133b 通过靶向 EZH2 抑制 Wnt/β-catenin 信号通路抑制神经胶质瘤进展。
Stem Cell Res Ther. 2019 Dec 16;10(1):381. doi: 10.1186/s13287-019-1446-z.
4
Serum exosomal miR-301a as a potential diagnostic and prognostic biomarker for human glioma.血清外泌体 miR-301a 作为人类神经胶质瘤潜在的诊断和预后生物标志物。
Cell Oncol (Dordr). 2018 Feb;41(1):25-33. doi: 10.1007/s13402-017-0355-3. Epub 2017 Oct 26.
5
FAM3C Regulates Glioma Cell Proliferation, Invasion, Apoptosis, and Epithelial Mesenchymal Transition via the Notch Pathway.FAM3C通过Notch信号通路调控胶质瘤细胞的增殖、侵袭、凋亡及上皮-间质转化。
Cancer Med. 2024 Dec;13(23):e70412. doi: 10.1002/cam4.70412.
6
Identifying Differentially Expressed tRNA-Derived Small Fragments as a Biomarker for the Progression and Metastasis of Colorectal Cancer.鉴定差异表达的 tRNA 衍生的小片段作为结直肠癌进展和转移的生物标志物。
Dis Markers. 2022 Jan 6;2022:2646173. doi: 10.1155/2022/2646173. eCollection 2022.
7
Effects of spinal cord injury associated exosomes delivered tRF-41 on the progression of spinal cord injury progression.脊髓损伤相关外泌体传递的 tRF-41 对脊髓损伤进展的影响。
Genomics. 2024 Jul;116(4):110885. doi: 10.1016/j.ygeno.2024.110885. Epub 2024 Jun 11.
8
Global profile of tRNA-derived small RNAs in gastric cancer patient plasma and identification of tRF-33-P4R8YP9LON4VDP as a new tumor suppressor.胃癌患者血浆中 tRNA 衍生的小 RNA 的全球特征分析及 tRF-33-P4R8YP9LON4VDP 作为新的肿瘤抑制因子的鉴定。
Int J Med Sci. 2021 Feb 4;18(7):1570-1579. doi: 10.7150/ijms.53220. eCollection 2021.
9
Dysregulated transfer RNA-derived small RNAs as potential gastric cancer biomarkers.失调的转运RNA衍生小RNA作为潜在的胃癌生物标志物
Exp Biol Med (Maywood). 2024 Dec 13;249:10170. doi: 10.3389/ebm.2024.10170. eCollection 2024.
10
Exosomal tRNA-derived small RNA as a promising biomarker for cancer diagnosis.外泌体 tRNA 衍生的小 RNA 作为癌症诊断有前景的生物标志物。
Mol Cancer. 2019 Apr 2;18(1):74. doi: 10.1186/s12943-019-1000-8.

本文引用的文献

1
Optimizing of a suitable protocol for isolating tissue-derived extracellular vesicles and profiling small RNA patterns in hepatocellular carcinoma.优化一种合适的方案以分离肝癌组织来源的细胞外囊泡并分析其小 RNA 图谱。
Liver Int. 2024 Oct;44(10):2672-2686. doi: 10.1111/liv.16011. Epub 2024 Jul 22.
2
The Emerging Function and Promise of tRNA-Derived Small RNAs in Cancer.tRNA衍生的小RNA在癌症中的新兴功能与前景
J Cancer. 2024 Jan 27;15(6):1642-1656. doi: 10.7150/jca.89219. eCollection 2024.
3
Multiple regulatory roles of the transfer RNA-derived small RNAs in cancers.
转运RNA衍生的小RNA在癌症中的多种调控作用。
Genes Dis. 2023 Apr 13;11(2):597-613. doi: 10.1016/j.gendis.2023.02.053. eCollection 2024 Mar.
4
Emerging roles of tRNA-derived small RNAs in cancer biology.tRNA 衍生的小 RNA 在癌症生物学中的新兴作用。
Exp Mol Med. 2023 Jul;55(7):1293-1304. doi: 10.1038/s12276-023-01038-5. Epub 2023 Jul 10.
5
Effect of miR‑29a‑3p in exosomes on glioma cells by regulating the PI3K/AKT/HIF‑1α pathway.外泌体 miR-29a-3p 通过调控 PI3K/AKT/HIF-1α 通路对胶质瘤细胞的影响。
Mol Med Rep. 2023 Mar;27(3). doi: 10.3892/mmr.2023.12959. Epub 2023 Feb 17.
6
Hypoxic glioma cell-secreted exosomal circ101491 promotes the progression of glioma by regulating miR-125b-5p/EDN1.缺氧胶质瘤细胞分泌的外泌体circ101491通过调控miR-125b-5p/EDN1促进胶质瘤进展。
Brain Res Bull. 2023 Apr;195:55-65. doi: 10.1016/j.brainresbull.2023.02.006. Epub 2023 Feb 14.
7
An old friend with a new face: tRNA-derived small RNAs with big regulatory potential in cancer biology.旧友新颜：tRNA 衍生的小 RNA 在癌症生物学中具有巨大的调控潜力。
Br J Cancer. 2023 May;128(9):1625-1635. doi: 10.1038/s41416-023-02191-4. Epub 2023 Feb 9.
8
Transfer RNA-derived small RNAs (tsRNAs) sequencing revealed a differential expression landscape of tsRNAs between glioblastoma and low-grade glioma.转运RNA衍生的小RNA（tsRNAs）测序揭示了胶质母细胞瘤和低级别胶质瘤之间tsRNAs的差异表达图谱。
Gene. 2023 Mar 1;855:147114. doi: 10.1016/j.gene.2022.147114. Epub 2022 Dec 13.
9
The tRNA-Cys-GCA Derived tsRNAs Suppress Tumor Progression of Gliomas via Regulating VAV2.tRNA-Cys-GCA 衍生的 tsRNAs 通过调控 VAV2 抑制神经胶质瘤的肿瘤进展。
Dis Markers. 2022 Nov 15;2022:8708312. doi: 10.1155/2022/8708312. eCollection 2022.
10
Clinical Characterization and Prognostic Value of TPM4 and Its Correlation with Epithelial-Mesenchymal Transition in Glioma.TPM4在胶质瘤中的临床特征、预后价值及其与上皮-间质转化的相关性
Brain Sci. 2022 Aug 24;12(9):1120. doi: 10.3390/brainsci12091120.