Izdebski Radosław, Biedrzycka Marta, Urbanowicz Paweł, Żabicka Dorota, Gniadkowski Marek
Department of Molecular Microbiology, National Medicines Institute, Warsaw, Poland.
Department of Epidemiology and Clinical Microbiology, National Medicines Institute, Warsaw, Poland.
Eur J Clin Microbiol Infect Dis. 2025 Apr 9. doi: 10.1007/s10096-025-05118-4.
This study focused on genomic epidemiology of metallo-β-lactamase (MBL)-producing Citrobacter spp. in Poland. It included 118 isolates with VIM (n = 100), NDM (n = 17) or IMP (n = 1) enzymes from 2011 to 19, largely C. freundii (n = 94) of 33 sequence types (STs). Three globally-spread STs were more prevalent, namely ST18 (n = 15), ST8 (n = 11) and ST22 (n = 10), each diversified clonally and by VIM and NDM determinants. Only few outbreaks were evidenced, indicating MBL presence to result mainly from independent acquisitions, consistent with co-identifications of Citrobacter isolates (n = 12) with other MBL-producing Enterobacterales. Despite lower outbreak potential, Citrobacter spp. constitute a significant MBL reservoir, requiring attention and surveillance.
本研究聚焦于波兰产金属β-内酰胺酶（MBL）的柠檬酸杆菌属细菌的基因组流行病学。研究涵盖了2011年至2019年间分离出的118株携带VIM（n = 100）、NDM（n = 17）或IMP（n = 1）酶的菌株，主要为弗氏柠檬酸杆菌（n = 94），分属于33种序列类型（STs）。三种全球传播的STs更为常见，即ST18（n = 15）、ST8（n = 11）和ST22（n = 10），每种类型在克隆水平以及VIM和NDM决定簇方面都具有多样性。仅证实了少数几起暴发事件，表明MBL的存在主要源于独立获得，这与柠檬酸杆菌分离株（n = 12）与其他产MBL肠杆菌科细菌的共同鉴定结果一致。尽管暴发潜力较低，但柠檬酸杆菌属细菌构成了一个重要的MBL储存库，需要予以关注和监测。
