Smaal E B, Romijn D, Geurts van Kessel W S, de Kruijff B, de Gier J
J Lipid Res. 1985 May;26(5):634-7.
A simple and efficient procedure is described for the preparation of cardiolipin sodium salt from beef heart. A crude phospholipid fraction is isolated by chloroform-methanol extraction of the homogenized tissue, followed by acetone precipitation and reprecipitation in 4% aqueous CaCl2-methanol. Cardiolipin is separated from the calcium salts of the acidic phospholipids by partition column chromatography on silica gel (Polygosil 60-63100) using 2-propanol-cyclohexane-water 50:43:7 (v/v/v) as eluent. Further purification of the cardiolipin is achieved by high performance liquid chromatography of the calcium salt on silica gel (Lichrosorb Si 60-5) with a neutral eluent (2-propanol-cyclohexane-water 45:50:5 (v/v/v], followed by quantitative conversion to the sodium salt. The yield of this procedure is 1.5-2.1 g of pure 99% sodium salt of cardiolipin per kg of moist ventricular tissue.
本文描述了一种从牛心制备心磷脂钠盐的简单高效方法。通过用氯仿 - 甲醇萃取匀浆组织来分离粗磷脂部分，随后进行丙酮沉淀，并在4%的氯化钙 - 甲醇水溶液中再沉淀。使用2 - 丙醇 - 环己烷 - 水50:43:7（v/v/v）作为洗脱剂，通过硅胶（Polygosil 60 - 63100）分配柱色谱法将心磷脂与酸性磷脂的钙盐分离。通过在硅胶（Lichrosorb Si 60 - 5）上用中性洗脱剂（2 - 丙醇 - 环己烷 - 水45:50:5（v/v/v）对钙盐进行高效液相色谱法进一步纯化心磷脂，随后定量转化为钠盐。该方法的产率为每千克湿心室组织可得到1.5 - 2.1克纯度为纯99%的心磷脂钠盐。
