Teng J I, Smith L L
J Chromatogr. 1985 Apr 12;339(1):35-44. doi: 10.1016/s0378-4347(00)84625-3.
Resolution of freshly prepared and of commercially available (degraded) samples of cardiolipin into 15-30 components has been accomplished by reversed-phase high-performance liquid chromatography using a 3-micron particulate Microsorb C18 column irrigated with linear gradients of acetonitrile--methanol--10 mM phosphate buffer pH 7.4. Selected resolved components were crystallized and characterized by infrared absorption spectra. Saponification of other components and identification of component fatty acids by reversed-phase high-performance liquid chromatography demonstrated the presence of ten fatty acids (14:0, 14:1, 16:0, 16:1, 18:0, 18:1, 18:2, 18:3, 20:0, 20:4), with linoleic acid (18:2) identified in all resolved components. From fatty acid composition data it appears that several resolved fractions consist of single cardiolipin molecular species.
通过反相高效液相色谱法，使用3微米微粒的Microsorb C18柱，用乙腈-甲醇-10 mM pH 7.4磷酸盐缓冲液的线性梯度冲洗，已将新制备的和市售的（降解的）心磷脂样品分离成15 - 30个组分。选择的分离组分被结晶并通过红外吸收光谱进行表征。其他组分的皂化以及通过反相高效液相色谱法对组分脂肪酸的鉴定表明存在十种脂肪酸（14:0、14:1、16:0、16:1、18:0、18:1、18:2、18:3、20:0、20:4），在所有分离组分中均鉴定出亚油酸（18:2）。从脂肪酸组成数据来看，几个分离馏分似乎由单一的心磷脂分子种类组成。
