Wang Mengya, Jiang Hailin, Wang Chuhan, Zhao Chunyan, Li Jinghua, Sun Yanbo, Yu Xin, Huang Honglan
Department of Pathogen Biology, College of Basic Medical Sciences, Jilin University, Changchun, China.
Arch Virol. 2025 Apr 9;170(5):95. doi: 10.1007/s00705-025-06281-x.
Abuse of antibiotics has led to increased rates of resistance in extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae and an acceleration in the emergence of drug-resistant strains, which can have serious consequences for nosocomial infections. In this study, phage MY02, which infects ESBL-producing Klebsiella pneumoniae, was isolated from sewage and characterized. Phage MY02 was found to have an optimal multiplicity of infection of 0.001, with a lysis period of up to 40 minutes and an average burst of about 80 plaque-forming units per cell. The phage was found to be stable over a temperature range of -20 to 60°C and a pH range of 3-11 and to have a broad host range. Whole-genome sequencing showed that the genome of phage MY02 is ??171,821?? bp in length and contains 293 open reading frames. Sequence comparisons and phylogenetic analysis showed that phage MY02 belongs to the genus Marfavirus in the class Caudoviricetes. This novel broad-spectrum Klebsiella pneumoniae phage has potential applications against bacterial infections.
抗生素的滥用导致产超广谱β-内酰胺酶（ESBL）的肺炎克雷伯菌耐药率上升，耐药菌株的出现加速，这可能对医院感染产生严重后果。在本研究中，从污水中分离并鉴定了感染产ESBL肺炎克雷伯菌的噬菌体MY02。发现噬菌体MY02的最佳感染复数为0.001，裂解期长达40分钟，每个细胞平均爆发约80个噬菌斑形成单位。该噬菌体在-20至60°C的温度范围和3-11的pH范围内稳定，且宿主范围广泛。全基因组测序表明，噬菌体MY02的基因组长度为171,821 bp，包含293个开放阅读框。序列比较和系统发育分析表明，噬菌体MY02属于长尾噬菌体科的马尔法病毒属。这种新型广谱肺炎克雷伯菌噬菌体在对抗细菌感染方面具有潜在应用价值。
