Gan Ying, Li Zheng, Ruan Mingjian, Yang Yang
Department of Urology, Peking University First Hospital, Beijing, 100034, China.
Institute of Urology, Peking University, Beijing, 100034, China.
BMC Urol. 2025 Apr 9;25(1):82. doi: 10.1186/s12894-025-01767-x.
Clear cell renal cell carcinoma (ccRCC) is fundamentally a metabolic disease. To investigate the underlying metabolite changes in the occurrence of ccRCC, we analyzed untargeted metabolomics of 15 ccRCC samples and paired adjacent non-malignant renal tissues by UHPLC-MS/MS analyses. In this study, 511 differential metabolites were screened, of which the top ten up-regulated metabolites in ccRCC were histamine, 1-methylnicotinamide, L-kynurenine, cortisol, tetrahydrocorticosterone, calcitriol, D-erythrose 4-phosphate, citric acid, sedoheptulose 1,7-bisphosphate, and UDP-alpha-D-galactose, and the top down-regulated metabolites were D-cysteine, acetylcholine, pantothenic acid, cytosine, UMP, biocytin, dUMP, 5-phosphoribosyl 1-pyrophosphate, cytidine-5'-monophosphate, and 16α-hydroxyestrone. KEGG pathways enrichment analysis further demonstrated several highlighted pathways: steroid hormone biosynthesis, pyrimidine metabolism, and vitamin digestion and absorption. Our study reveals metabolic patterns of ccRCC and provides insight into the potential biomarker panel to diagnose ccRCC.
透明细胞肾细胞癌（ccRCC）本质上是一种代谢性疾病。为了研究ccRCC发生过程中潜在的代谢物变化，我们通过超高效液相色谱-串联质谱（UHPLC-MS/MS）分析对15例ccRCC样本及其配对的相邻非恶性肾组织进行了非靶向代谢组学分析。在本研究中，筛选出511种差异代谢物，其中ccRCC中上调排名前十的代谢物为组胺、1-甲基烟酰胺、L-犬尿氨酸、皮质醇、四氢皮质酮、骨化三醇、D-赤藓糖-4-磷酸、柠檬酸、景天庚酮糖-1,7-二磷酸和UDP-α-D-半乳糖，下调排名前十的代谢物为D-半胱氨酸、乙酰胆碱、泛酸、胞嘧啶、尿苷一磷酸、生物胞素、脱氧尿苷一磷酸、5-磷酸核糖-1-焦磷酸、胞苷-5'-单磷酸和16α-羟基雌酮。京都基因与基因组百科全书（KEGG）通路富集分析进一步显示了几个突出的通路：类固醇激素生物合成、嘧啶代谢以及维生素消化与吸收。我们的研究揭示了ccRCC的代谢模式，并为诊断ccRCC的潜在生物标志物组提供了见解。
