Lombardo Jeremy A, Banyard Derek A, Zalazar David, Ziegler Mary, Sorensen Alexandria M, Phummirat Pisrut, Widgerow Alan D, Haun Jered B
Aesthet Surg J. 2025 Jul 15;45(8):850-859. doi: 10.1093/asj/sjaf055.
Mechanical processing of lipoaspirate (LA) produces a stromal vascular fraction (SVF) without enzymatic digestion for use in aesthetic, surgical, and regenerative applications. We recently presented novel device technologies that increased mesenchymal stem cell (MSC) content relative to standard nanofat (NF) processing.
Here, we introduce a third technology designed to enhance fluid shear forces and explore the impact of mechanical processing on regenerative potential in vitro.
Human LA samples were processed with our previously reported emulsification micronization device and filtration device, and then optimized using a new shearing device (SD). Results were analyzed for total cell count, viability, and percentages of endothelial progenitor cells (EPCs) and MSCs compared to manual NF processing, both immediately and following 24-hour culture. Expression of genes related to wound healing was quantified by real-time quantitative polymerase chain reaction, and angiogenic capacity was determined with an in vitro 3-dimensional sprouting assay.
The SD did not significantly affect MSC recovery or viability, but EPCs were enriched in a shear stress-dependent manner. Gene expression was not altered immediately after processing, but after culture we noted changes to wound-healing transcriptional programs that were consistently stronger for our devices than NF. Differences were statistically significant for CXCL1, IL1β, IL6, CSF3, and COL1A2. Notably, angiogenic vessel sprouting was significantly enhanced for our devices compared to NF.
Mechanical processing of lipoaspirate with our 3-device platform resulted in greater enrichment of stem and progenitor cells, activation of genes implicated in wound healing, and induction of angiogenesis in vitro relative to NF. Future studies will ascertain potential implications in vivo for all indications that currently utilize NF, as well as automate the process within an integrated system.
脂肪抽吸物（LA）的机械处理可产生无需酶消化的基质血管成分（SVF），用于美容、外科和再生应用。我们最近展示了一些新型设备技术，这些技术相对于标准纳米脂肪（NF）处理增加了间充质干细胞（MSC）的含量。
在此，我们介绍第三种旨在增强流体剪切力的技术，并探讨机械处理对体外再生潜力的影响。
使用我们先前报道的乳化微粉化装置和过滤装置对人LA样本进行处理，然后使用一种新的剪切装置（SD）进行优化。与手动NF处理相比，在处理后立即以及培养24小时后，分析总细胞计数、活力以及内皮祖细胞（EPC）和MSC的百分比。通过实时定量聚合酶链反应对与伤口愈合相关的基因表达进行定量，并通过体外三维发芽试验测定血管生成能力。
SD对MSC的回收率或活力没有显著影响，但EPC以剪切应力依赖的方式富集。处理后基因表达没有立即改变，但培养后我们注意到伤口愈合转录程序发生了变化，我们的装置的变化始终比NF更强。CXCL1、IL1β、IL6、CSF3和COL1A2的差异具有统计学意义。值得注意的是，与NF相比，我们的装置的血管生成血管发芽显著增强。
与NF相比，使用我们的三装置平台对脂肪抽吸物进行机械处理可使干细胞和祖细胞更富集，激活与伤口愈合相关的基因，并在体外诱导血管生成。未来的研究将确定目前使用NF的所有适应症在体内的潜在影响，并在集成系统内实现该过程的自动化。
