Li Fang, Xiong Shuyue, Tan Yijun, Luo Mingming, Wu Zijian
School of Information Engineering, Tianjin University of Commerce Tianjin China
Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce Tianjin China
RSC Adv. 2025 Apr 9;15(14):11271-11282. doi: 10.1039/d5ra01006c. eCollection 2025 Apr 4.
Antibiotics play an excellent role in preventing and treating animal diseases, but their improper use poses a potential threat to public health. Designing a sensing platform to detect trace amounts of antibiotic residues in actual samples is a significant challenge. To achieve this objective, a self-assembly aptasensor based on a two-round signal amplification strategy for kanamycin (KANA) trace detection was demonstrated by employing carbon dot (CD) decorated TiC MXene as electrode modification material and the complex of double-stranded DNA (dsDNA) and methylene blue (MB) as probes, where dsDNA was composed of complementary DNA (cDNA) and aptamers. The CDs can effectively suppress the self-stacking of TiC MXene to promote electron transfer and provide plenty of exposed active sites for the aptamer to capture KANA precisely. The MB inserted into dsDNA would be liberated upon interaction with KANA due to a competitive process that occurs among cDNA and KANA, reducing electrical signal. Under the optimal conditions, the constructed aptasensor exhibited a good linear relationship between the output signal and the logarithm of KANA concentration in the range of 1 fM-1 mM, and the limit of detection is 0.892 fM. The satisfactory selectivity, stability, and reproducibility suggested that the prepared aptasensor can be a universal platform to detect other antibiotic residues by anchoring corresponding aptamers. Furthermore, it has been successfully applied to determine KANA in milk samples (recovery rates ranged from 101.01% to 107.21%, and the RSD was below 5%), demonstrating potential application prospects in the food-safety analysis field.
抗生素在预防和治疗动物疾病方面发挥着出色的作用，但其不当使用对公众健康构成潜在威胁。设计一个传感平台来检测实际样品中的痕量抗生素残留是一项重大挑战。为实现这一目标，通过采用碳点（CD）修饰的TiC MXene作为电极修饰材料以及双链DNA（dsDNA）与亚甲基蓝（MB）的复合物作为探针，展示了一种基于两轮信号放大策略的用于卡那霉素（KANA）痕量检测的自组装适体传感器，其中dsDNA由互补DNA（cDNA）和适体组成。碳点可以有效抑制TiC MXene的自堆积以促进电子转移，并为适体精确捕获KANA提供大量暴露的活性位点。由于cDNA和KANA之间发生的竞争过程，插入dsDNA中的MB在与KANA相互作用时会被释放，从而降低电信号。在最佳条件下，构建的适体传感器在1 fM - 1 mM范围内输出信号与KANA浓度的对数之间呈现良好的线性关系，检测限为0.892 fM。令人满意的选择性、稳定性和重现性表明，通过锚定相应的适体，制备的适体传感器可以成为检测其他抗生素残留的通用平台。此外，它已成功应用于牛奶样品中KANA的测定（回收率在101.01%至107.21%之间，相对标准偏差低于5%），在食品安全分析领域展示了潜在的应用前景。
