Cui Xiaoying, Zhao Ke, Qu Zhengquan, Chao Xipeng, Xie Lingling, Chen Hanyu, He Baoshan, Zhang Baozhong
School of Environmental Engineering, Henan University of Technology, Lianhua Road 100#, Zhengzhou 450001, Henan Province, People's Republic of China.
School of Food Science and Technology, Henan University of Technology, Lianhua Road 100#, Zhengzhou 450001, Henan Province, People's Republic of China.
Bioelectrochemistry. 2025 Jun;163:108881. doi: 10.1016/j.bioelechem.2024.108881. Epub 2024 Dec 9.
In this work, an efficient sensing platform deoxynivalenol (DON) detection was constructed through monitoring the current change of a competitive mechanism triggered by DON, leading the signal label detached from the electrode surface by square-wave voltammetry using thionine (Thi) as a redox indicator. The complementary strand of aptamer (cDNA) and Thi were loaded onto Fe/Ni bimetallic metal-organic framework loaded with sliver nanoparticles (AgNPs@FeNi-MOF) to construct AgNPs@FeNi-MOF/cDNA/Thi signal probes. In the presence of DON, the aptamer sequence was more predisposed to form an aptamer-DON complex, resulting in the displacement of the cDNA. The signal probe was subsequently released, leading to a decrease in the signal intensity of Thi. Notably, AgNPs@FeNi-MOF has a larger electroactive specific surface area and is able to load more cDNA and thi, which can amplify the signal. Under the optimal experimental conditions, the developed sensor exhibits a good linear response in the range of 1 × 10 to 1 × 10 pg/mL, with a limit of detection (LOD) of 5.68 fg/mL and has good selectivity, reproducibility and stability.
在本工作中，通过监测由脱氧雪腐镰刀菌烯醇（DON）触发的竞争机制的电流变化，构建了一种高效的DON传感平台，利用硫堇（Thi）作为氧化还原指示剂，通过方波伏安法使信号标签从电极表面脱离。将适配体互补链（cDNA）和Thi负载到负载有银纳米颗粒的铁/镍双金属金属有机框架（AgNPs@FeNi-MOF）上，构建AgNPs@FeNi-MOF/cDNA/Thi信号探针。在DON存在的情况下，适配体序列更倾向于形成适配体-DON复合物，导致cDNA被取代。随后信号探针被释放，导致Thi的信号强度降低。值得注意的是，AgNPs@FeNi-MOF具有更大的电活性比表面积，能够负载更多的cDNA和Thi，从而可以放大信号。在最佳实验条件下，所开发的传感器在1×10至1×10 pg/mL范围内表现出良好的线性响应，检测限（LOD）为5.68 fg/mL，具有良好的选择性、重现性和稳定性。
