Miescher Iris, Wolint Petra, Opelz Christine, Snedeker Jess G, Giovanoli Pietro, Calcagni Maurizio, Buschmann Johanna
Division of Plastic Surgery and Hand Surgery, University Hospital Zurich, Sternwartstrasse 14, 8091 Zurich, Switzerland.
University Clinic Balgrist, Orthopaedic Biomechanics, Forchstrasse 340, 8008 Zurich, Switzerland.
Data Brief. 2025 Mar 15;60:111459. doi: 10.1016/j.dib.2025.111459. eCollection 2025 Jun.
Hyaluronic acid (HA) is a natural bio macromolecule and a good bio lubricant. As it is a predominant component of the synovial fluid, the aim was to explore the effects HA on rabbit Achilles tenocytes in vitro. The data set presents proliferation assessed by the Alamar Blue® assay; and gene expression of tenocytes that were cultured either with or without supplementation of high-molecular weight HA in a concentration of 1.4 mg/mL. There was a daily medium exchange with fresh HA supplementation. On days 3, 7 and 14 the following target genes were analysed and manifold induction was calculated against the control (no HA): Biglycan, Decorin, Aggrecan, Collagen 1A1, Collagen 1A2, Collagen 3, LOX, IL-6, TNF-α, PAR-2, ALOX-15, Tenascin-C, Tenomodulin, Mohawk, α-SMA, TIMP-1, MMP-2, MMP-9, and ki67. These data might be interesting for researchers working in the tendon field.
透明质酸（HA）是一种天然生物大分子，也是一种优质的生物润滑剂。由于它是滑液的主要成分，因此目的是在体外探索HA对兔跟腱细胞的影响。该数据集展示了通过Alamar Blue®检测法评估的增殖情况；以及在添加或不添加浓度为1.4 mg/mL的高分子量HA的条件下培养的腱细胞的基因表达。每天更换培养基并补充新鲜的HA。在第3、7和14天，分析以下靶基因，并计算相对于对照组（无HA）的多重诱导倍数：双糖链蛋白聚糖、核心蛋白聚糖、聚集蛋白聚糖、胶原蛋白1A1、胶原蛋白1A2、胶原蛋白3、赖氨氧化酶、白细胞介素-6、肿瘤坏死因子-α、蛋白酶激活受体-2、脂氧合酶-15、腱生蛋白-C、肌腱调节蛋白、莫霍克蛋白、α-平滑肌肌动蛋白、金属蛋白酶组织抑制因子-1、基质金属蛋白酶-2、基质金属蛋白酶-9和增殖细胞核抗原。这些数据可能会引起从事肌腱领域研究的人员的兴趣。
