Ballas S K, Tabbara K F, Murphy D L, Mohandas N, Clark M R, Shohet S B
J Clin Lab Immunol. 1985 Apr;16(4):217-22.
NZB and B/W hybrid mice develop compensated hemolytic anemia during the first year of their life. By the age of 3-5 months, their erythrocytes show evidence of spherocytosis, increased osmotic fragility and decreased whole cell deformability, as measured by ektacytometry, a laser diffraction technique. The presence of spherocytes with decreased surface area/volume ratio was confirmed by scanning electron microscopy and osmotic gradient ektacytometry. Whereas these abnormalities persisted and worsened in the NZB mice with further growth, they gradually improved and reverted to normal by the age of 12 months in B/W mice. This spontaneous improvement seems to be due to the accumulation of red cell membrane lipids reflecting the lipemia of immune complex nephritis in B/W mice. The implications of these findings in the modulation of autoimmune hemolytic anemia are discussed.
NZB和B/W杂交小鼠在出生后的第一年发生代偿性溶血性贫血。到3 - 5月龄时，通过激光衍射技术——激光衍射血细胞分析法测量，它们的红细胞呈现出球形红细胞增多、渗透脆性增加和全细胞变形性降低的迹象。通过扫描电子显微镜和渗透梯度激光衍射血细胞分析法证实了表面积/体积比降低的球形红细胞的存在。在NZB小鼠中，随着进一步生长，这些异常持续存在且恶化，而在B/W小鼠中，到12月龄时这些异常逐渐改善并恢复正常。这种自发改善似乎是由于反映B/W小鼠免疫复合物性肾炎脂血症的红细胞膜脂质积累所致。本文讨论了这些发现对自身免疫性溶血性贫血调节的意义。
