Ong Victor, Mohamed Mona A, Ma Haozheng, Al-Shami Abdulrahman, Khazaee Nejad Sina, Amirghasemi Farbod, Tabassum Asna, Lee Michael Jayden, Rohleder Alexander, Zhu Cathy, Tam Chur, Nowlen Patrick, Mousavi Maral P S
Alfred E. Mann Department of Biomedical Engineering, University of Southern California, 1042 Downey Way, STE 140, Los Angeles, 90089, CA, USA.
Alfred E. Mann Department of Biomedical Engineering, University of Southern California, 1042 Downey Way, STE 140, Los Angeles, 90089, CA, USA; Department of Chemistry, University of Southern California, 3620 McClintock Ave, Los Angeles 90089, CA, USA; Keck School of Medicine, University of Southern California, 1975 Zonal Ave, Los Angeles 90033, CA, USA; Alfred E. Mann School of Pharmacy and Pharmaceutical Sciences, University of Southern California, 1985 Zonal Avenue, Los Angeles 90089, CA, USA.
Biosens Bioelectron. 2025 Jul 15;280:117386. doi: 10.1016/j.bios.2025.117386. Epub 2025 Apr 3.
Bilirubin (BR) in blood serum is elevated during jaundice (a common complication among newborns). Accessible and frequent monitoring of BR is necessary for timely diagnosis and intervention. This work developed a low-cost electrochemical sensor for point-of-care and at-home measurement of BR. The sensor measures BR based on its oxidation at a laser-induced graphene electrode. A microfluidic channel for sample wicking is embedded on the electrodes to bifurcate the blood sample into two zones to allow measurement of different BR forms (conjugated and unconjugated). One zone remains bare, while the second zone is impregnated with caffeine sodium benzoate, which acts as an accelerant to release albumin-bound BR and makes it accessible for electrochemical oxidation. The sensors demonstrate linear response in the concentration range of 100 to 500 µmolL total bilirubin (4:1 ratio of unconjugated to conjugated bilirubin) in undiluted blood serum. Physiologically relevant BR levels range from 170 µmolL (healthy concentrations) to 460 µmolL (elevated, unhealthy concentration). Recovery values at 150 and 250 µmolL are 119 % and 104 %, which fall within the 20 % error required by the United States Food and Drug Administration. This device represents the first point-of-care bilirubin-sensor capable of distinguishing between different forms of bilirubin.
黄疸（新生儿常见并发症）期间血清胆红素（BR）会升高。为了及时诊断和干预，对BR进行便捷且频繁的监测很有必要。这项工作开发了一种低成本的电化学传感器，用于即时护理和家庭环境下BR的测量。该传感器基于BR在激光诱导石墨烯电极上的氧化反应来测量BR。电极上嵌入了一个用于样品吸液的微流控通道，将血液样本分成两个区域，以便测量不同形式的BR（结合型和非结合型）。一个区域保持裸露，而第二个区域浸渍有苯甲酸钠咖啡因，其作为促进剂可释放与白蛋白结合的BR，使其能够进行电化学氧化。该传感器在未稀释血清中总胆红素浓度范围为100至500 µmol/L（非结合型与结合型胆红素比例为4:1）时呈现线性响应。生理相关的BR水平范围从170 µmol/L（健康浓度）到460 µmol/L（升高的、不健康浓度）。150和250 µmol/L时的回收率分别为119%和104%，均在美国食品药品监督管理局要求的20%误差范围内。该设备是首个能够区分不同形式胆红素的即时护理胆红素传感器。
