Abundance and Composition of the Meconium Microbiota in Preterm Infants with Infections, Feeding Intolerance, or Necrotizing Enterocolitis.

BACKGROUND

The role of the microbial flora of the gut of a newborn is of scientific and practical interest. The aim of this study was to assess the abundance and composition of the meconium microbiota in preterm infants with infections, feeding intolerance, or necrotizing enterocolitis (NEC).

METHODS

Eighty-four preterm infants born by cesarean section were prospectively enrolled in this study. Out of the 28 diseased infants, 23 developed infections, including 8 cases of sepsis, 10 cases of pneumonia, 1 case of enterocolitis, and 4 cases of NEC. Fifty-six (66.67%) preterm infants without these characteristics served as control group. General clinical information (gender, gestational age, birth weight, presence of preterm rupture of mem-branes, Apgar 1-minute score, and duration of hospitalization) was collected. First-pass meconium samples were collected for 16S rRNA microbiological analysis.

RESULTS

Compared with the control group, the diseased infants had a lower gestational age (p < 0.001) and lower body weight (p = 0.014). In addition, the hospitalization time of the diseased infants was longer than that of the control group (p < 0.001). On the α-diversity measure, there was no difference in species abundance and diversity between the two groups; on the β-diversity measure, the differences in the microbial composition of the two groups were subjected to PCoA analyses, which showed that there was a difference between the disease group and the control group. At the phylum level, the dominant phylum in both groups was p_Proteobacteria, with higher abundance of p_Firmicutes in the disease group. At the genus level, the dominant genus in both groups was g_Novosphingobium. Microbiome phenotype prediction by BugBase revealed that microbial phenotypes 'Gram-positive' and 'Anaerobic' were abundantly increased in the disease group; microbial function prediction did not differ between the two groups in terms of significant function.

CONCLUSIONS

The impact of infections, feeding intolerance, and NEC on a host is complex. Preterm infants delivered by cesarean section have p_Proteobacteria as the dominant phylum, with a higher abundance of p_Firmicutes in the disease group, a difference contributed by g_Peptoniphilus.