Jinan University, Guangzhou, Guangdong, 510000, China.
Department of Pediatrics, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, 524000, China.
J Microbiol. 2022 Oct;60(10):1021-1031. doi: 10.1007/s12275-022-2180-2. Epub 2022 Aug 19.
The potential role of the gut microbiota in the pathogenesis of feeding intolerance (FI) remains unclear. Understanding the role of the gut microbiota could provide a new avenue for microbiota-targeted therapeutics. This study aimed to explore the associations between aberrant gut microbiota and FI in very low or extremely low birth weight (VLBW/ELBW) preterm infants. In this observational case-control study, VLBW/ELBW infants were divided into two groups: FI group and feeding tolerance (FT) group. 16S rRNA gene sequencing was performed to analyze the gut microbial diversity and composition of the infants. The differences in the gut microbiota of the two groups were compared. In total, 165 stool samples were obtained from 44 infants, among which, 31 developed FI and 13 served as controls. Alpha diversity was the highest in the meconium samples of the two groups. LEfSe analysis revealed that the abundances of Peptostreptococcaceae, Clostridiales and Clostridia in the FT group were significantly higher than in the FI group. At the phylum level, the FI group was dominated by Proteobacteria, and the FT group was dominated by Firmicutes. The meconium samples of the FI group had higher proportions of γ-proteobacteria and Escherichia-Shigella and a lower proportion of Bacteroides compared with the FT group. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis demonstrated that aberrant gut bacteria in the FI group were strongly associated with dysregulation of C5-Branched-dibasic-acid-metabolism, protein kinases, and sporulation. These findings reveal candidate microbial markers to prevent FI. Increased relative abundances of γ-proteobacteria and Escherichia-Shigella and decreased abundance of Bacteroides in meconium were associated with an increased risk of FI, while Peptostreptococcaceae, Clostridiales and Clostridia reduced the risk of FI in VLBW/ELBW infants.
肠道微生物群在喂养不耐受（FI）发病机制中的潜在作用尚不清楚。了解肠道微生物群的作用可能为靶向微生物群的治疗提供新途径。本研究旨在探讨极低出生体重（VLBW/ELBW）早产儿肠道微生物群异常与 FI 之间的关系。在这项观察性病例对照研究中，将 VLBW/ELBW 婴儿分为 FI 组和喂养耐受（FT）组。对婴儿的肠道微生物多样性和组成进行 16S rRNA 基因测序分析。比较两组之间的肠道微生物群差异。共从 44 名婴儿中获得 165 份粪便样本，其中 31 名婴儿发生 FI，13 名作为对照。两组胎粪样本的α多样性最高。LEfSe 分析显示，FT 组中 Peptostreptococcaceae、Clostridiales 和 Clostridia 的丰度明显高于 FI 组。在门水平上，FI 组以变形菌门为主，FT 组以厚壁菌门为主。与 FT 组相比，FI 组的胎粪样本中 γ-变形菌门和大肠杆菌-志贺氏菌的比例较高，拟杆菌的比例较低。京都基因与基因组百科全书（KEGG）分析表明，FI 组中异常肠道细菌与 C5-支链二羧酸代谢、蛋白激酶和孢子形成的失调密切相关。这些发现揭示了预防 FI 的候选微生物标志物。胎粪中 γ-变形菌门和大肠杆菌-志贺氏菌的相对丰度增加，拟杆菌的丰度降低，与 FI 风险增加相关，而 Peptostreptococcaceae、Clostridiales 和 Clostridia 降低了 VLBW/ELBW 婴儿 FI 的风险。
