Donoghue H D
Antimicrob Agents Chemother. 1977 Jan;11(1):34-7. doi: 10.1128/AAC.11.1.34.
Exposure of growing cultures of Bacillus megaterium C4A(-) to ethidium bromide or an elevated growth temperature was found to eliminate megacin C production. Ethidium bromide resulted in a cure rate of up to 13%. Growth at 43 degrees C gave a cure rate of up to 99%. Megacin C production was lost spontaneously at a rate of 4% or less. There was a greater rate of spontaneous loss of megacin Cx production by B. megaterium 337, up to 14%. Growth at 43 degrees C resulted in a cure rate of up to 24% in this organism. Reversion to the Meg(+) state by cured clones has never been demonstrated. These observations support the hypothesis that production of megacins C and Cx is plasmid mediated. Meg(-) clones adsorbed more megacin than either parent strain and were more susceptible to megacin action.
已发现，将巨大芽孢杆菌C4A(-)的生长培养物暴露于溴化乙锭或提高生长温度会消除巨霉素C的产生。溴化乙锭导致的治愈几率高达13%。在43℃下生长，治愈几率高达99%。巨霉素C的产生以4%或更低的速率自发丧失。巨大芽孢杆菌337的巨霉素Cx产生的自发丧失速率更高，高达14%。在该菌株中，43℃下生长导致的治愈几率高达24%。从未证明治愈的克隆会回复到Meg(+)状态。这些观察结果支持了巨霉素C和Cx的产生是由质粒介导的这一假说。Meg(-)克隆比任何一个亲本菌株吸附的巨霉素都更多，并且对巨霉素的作用更敏感。
