巨大芽孢杆菌产巨霉素质粒pBM309的缺失突变体
Deletion mutants of megacinogenic plasmid pBM309 from Bacillus megaterium.
作者信息
Riabchenko N F, Rostás K
出版信息
Gene. 1983 Nov;25(1):67-70. doi: 10.1016/0378-1119(83)90168-3.
PMID:6319232
Abstract
A 46.8-kb plasmid, pBM309, of Bacillus megaterium determines the production of a bacteriocin, megacin A, and confers immunity against this antibiotic on the host cells. The megacin A (megA) and megacin A-immunity (megAim) genes were mapped on the physical map of pBM309 by using its deletion derivatives. Both genes were isolated as a 10.6-kb PstI fragment and cloned in Bacillus subtilis vector plasmid pBD9 for expression in B. megaterium.
摘要
巨大芽孢杆菌的一个46.8 kb质粒pBM309决定了细菌素巨霉素A的产生，并赋予宿主细胞对这种抗生素的免疫力。通过使用其缺失衍生物，将巨霉素A（megA）和巨霉素A免疫（megAim）基因定位在pBM309的物理图谱上。这两个基因作为一个10.6 kb的PstI片段被分离出来，并克隆到枯草芽孢杆菌载体质粒pBD9中，以便在巨大芽孢杆菌中表达。
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