Nahar Shamsun, Mahamud A G M Sofi Uddin, Cho Ah Jin, Ashrafudoulla Md, Yu Jisu, Park Si Hong, Ha Sang-Do
Department of Food Safety and Regulatory Science, Chung-Ang University, Anseong-si, Gyeonggi-do, 17546, Republic of Korea.
GreenTech-Based Food Safety Research Group, Chung-Ang University, BK21 Four, 4726 Seodong-Daero, Anseong-si, Gyeonggi-do, 17546, Republic of Korea.
Curr Microbiol. 2025 Apr 10;82(6):240. doi: 10.1007/s00284-025-04200-6.
The biofilm-mediated persistence of Pseudomonas aeruginosa in the food and biomedical sectors is currently a global concern. In light of this challenge, this study investigated a preventive approach against P. aeruginosa biofilm formation using Flavourzyme, a food-grade peptidase, considering its antibiofilm potential. The results revealed that a co-culture comprising 300 µL/mL (1 × minimum inhibitory concentration [MIC]) of Flavourzyme could kill P. aeruginosa. On the MBEC™ biofilm-forming device, 0.125 × MIC of Flavourzyme inhibited > 4.5 log CFU/peg of biofilm. Cell motilities and the biosynthesis of quorum sensing (QS) molecules such as N-acyl-homoserine lactones (AHLs), including C-HSL, decreased significantly at 0.06 × MIC of Flavourzyme and became undetectable at 0.125 × MIC. Interestingly, while 0.03 × MIC of Flavourzyme elicited diverse expressions of QS and virulence-regulating genes, ≥ 0.06 × MIC of Flavourzyme remarkably suppressed the relative genomic expressions. This study proposes Flavourzyme as a potent antibiofilm agent against P. aeruginosa biofilms, recommending specific concentrations for effective use in food preservation.
铜绿假单胞菌在食品和生物医学领域通过生物膜介导的持续性目前是一个全球关注的问题。鉴于这一挑战，本研究使用食品级肽酶风味酶，考虑到其抗生物膜潜力，研究了一种针对铜绿假单胞菌生物膜形成的预防方法。结果表明，包含300 μL/mL（1×最低抑菌浓度[MIC]）风味酶的共培养物可以杀死铜绿假单胞菌。在MBEC™生物膜形成装置上，0.125×MIC的风味酶抑制了>4.5 log CFU/栓的生物膜。在0.06×MIC的风味酶作用下，细胞运动性以及群体感应（QS）分子如N-酰基高丝氨酸内酯（AHLs）（包括C-HSL）的生物合成显著降低，在0.125×MIC时变得无法检测到。有趣的是，虽然0.03×MIC的风味酶引发了QS和毒力调节基因的不同表达，但≥0.06×MIC的风味酶显著抑制了相对基因组表达。本研究提出风味酶作为一种针对铜绿假单胞菌生物膜的有效抗生物膜剂，并推荐了在食品保鲜中有效使用的特定浓度。
