Castro Márquez Stephanie O, Tóth Viktor R, Kolchanova Sofiia, Wolfsberger Walter W, Oleksyk Taras K
Department of Biological Sciences, Oakland University, Rochester, MI, USA.
Department of Biology, University of Puerto Rico - Mayagüez, Mayagüez, Puerto Rico.
Sci Rep. 2025 Apr 10;15(1):12224. doi: 10.1038/s41598-025-96558-7.
Lake Balaton, a large shallow freshwater lake in Hungary, exhibits diverse bacterioplankton communities influenced by various environmental factors. This study aims to evaluate the bacterial diversity in Lake Balaton using the long-read approach to 16 S rRNA gene sequencing. Water samples were collected from a wide network of 33 locations across the lake's four basins and analyzed for bacterial community composition. Sequencing results revealed a high taxonomic diversity with significant zonal variations. Dominant families included Comamonadaceae, Burkholderiaceae, and Methylophilaceae. Environmental parameters such as temperature, pH, and CDOM were found to significantly correlate with bacterial abundance and diversity. The study underscores the utility and portability of using the long-read sequencing technology in assessing microbial diversity and provides insights into the ecological dynamics of bacterioplankton in freshwater lakes.
巴拉顿湖是匈牙利的一个大型浅水淡水湖，其浮游细菌群落多样，受多种环境因素影响。本研究旨在采用长读长方法对16S rRNA基因进行测序，以评估巴拉顿湖的细菌多样性。从该湖四个流域的33个地点组成的广泛网络中采集水样，并分析细菌群落组成。测序结果显示出高度的分类多样性以及显著的区域差异。优势科包括丛毛单胞菌科、伯克霍尔德菌科和嗜甲基菌科。研究发现温度、pH值和有色溶解有机物等环境参数与细菌丰度和多样性显著相关。该研究强调了使用长读长测序技术评估微生物多样性的实用性和可移植性，并为淡水湖浮游细菌的生态动态提供了见解。
