三种马铃薯品种（Solanum tuberosum.L）特定愈伤组织的再生及其对干旱胁迫耐受性的研究。

Regeneration of selected callus of three potato cultivars (Solanum tuberosum.L) and studying their tolerance to drought stress.

作者信息

Laila Lama Abdullah, Zaid Salim Hussein, Al-Biski Fahed, Dakah Abdulkarim

机构信息

Department of Plant Biology, Faculty of Sciences, Damascus University, Damascus, Syria.

Researcher at the National Commission for Biotechnology, Damascus, Syria.

出版信息

BMC Plant Biol. 2025 Apr 10;25(1):460. doi: 10.1186/s12870-025-06512-y.

DOI:10.1186/s12870-025-06512-y

PMID:40211133

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11983820/

Abstract

Drought stress poses a significant threat to potato production in Syria, necessitating the development of resilient cultivars. This study, conducted at the Plant Biotechnology Laboratory of the National Commission for Biotechnology in Damascus, investigated the impact of drought stress on key growth parameters of regenerated somaclones derived from selected callus of three potato cultivars (Solanum tuberosum L.): Salvador, Yalas, and Arizona. The research revealed striking differences in regeneration efficiency among cultivars. Yalas exhibited the highest somaclone regeneration rate at 32.1% when cultured on medium supplemented with 25 mg·L⁻ adenine sulfate and 1.5 mg·L⁻ BAP. Salvador and Arizona followed with regeneration rates of 21.4% and 14.5%, respectively, using 15 mg·L⁻ adenine sulfate plus 1.5 mg·L⁻ BAP. Comprehensive analysis of growth indicators (including plant height, leaf number and area, and root number and length) under drought stress conditions identified superior drought-tolerant somaclones. Notably, Y3 and Y2 (Yalas), S3 and S6 (Salvador), and A1 (Arizona) demonstrated exceptional drought resilience across multiple growth parameters. These findings provide valuable insights for potato breeding programs aimed at enhancing drought tolerance, potentially mitigating the impact of water scarcity on potato production in Syria and similar arid regions.

摘要

干旱胁迫对叙利亚的马铃薯生产构成了重大威胁，因此有必要培育具有抗逆性的品种。本研究在大马士革国家生物技术委员会的植物生物技术实验室进行，调查了干旱胁迫对源自三个马铃薯品种（Solanum tuberosum L.）：萨尔瓦多、亚拉斯和亚利桑那州的选定愈伤组织再生体细胞克隆关键生长参数的影响。研究揭示了不同品种间再生效率存在显著差异。在添加25 mg·L⁻硫酸腺嘌呤和1.5 mg·L⁻ 苄氨基嘌呤的培养基上培养时，亚拉斯的体细胞克隆再生率最高，为32.1%。使用15 mg·L⁻硫酸腺嘌呤加1.5 mg·L⁻苄氨基嘌呤时，萨尔瓦多和亚利桑那州的再生率分别为21.4%和14.5%。对干旱胁迫条件下的生长指标（包括株高、叶片数量和面积以及根数和长度）进行综合分析，确定了耐旱性较强的体细胞克隆。值得注意的是，Y3和Y2（亚拉斯）、S3和S6（萨尔瓦多）以及A1（亚利桑那州）在多个生长参数上表现出卓越的抗旱能力。这些发现为旨在提高耐旱性的马铃薯育种计划提供了有价值的见解，有可能减轻水资源短缺对叙利亚及类似干旱地区马铃薯生产的影响。