Genç Vocational School, Forestry Department, Bingol University, Bingol, Turkey.
PeerJ. 2024 Mar 20;12:e17116. doi: 10.7717/peerj.17116. eCollection 2024.
The potato ( L.), an important field crop consumed extensively worldwide, is adversely affected by abiotic stress factors especially drought. Therefore, it is vital to understand the genetic mechanism under drought stress to decrease loose of yield and quality . This trial aimed to screen drought-responsive gene expressions of potato and determine the drought-tolerant potato cultivar. The trial pattern is a completely randomized block design (CRBD) with four replications under greenhouse conditions. Four cultivars (Brooke, Orwell, Vr808, Shc909) were irrigated with four different water regimes (control and three stress conditions), and the gene expression levels of 10 potato genes were investigated. The stress treatments as follows: Control = 100% field capacity; slight drought = 75% field capacity; moderate drought = 50% field capacity, and severe drought 25% field capacity. To understand the gene expression under drought stress in potato genotypes, RT-qPCR analysis was performed and results showed that the genes most associated with drought tolerance were the gene, , , , and . The gene had the lowest genetic expression in all cultivars. Among the cultivars, the Orwell exhibited the highest expression of the gene under drought stress. Overall, the cultivar with the highest gene expression was the Vr808, closely followed by the Brooke cultivar. As a result, it was determined that potato cultivars Orwell, Vr808, and Brooke could be used as parents in breeding programs to develop drought tolerant potato cultivars.
马铃薯（L.）是一种重要的大田作物，在全球范围内广泛消费，但其易受到非生物胁迫因素的影响，特别是干旱。因此，了解干旱胁迫下的遗传机制对于减少产量和品质的损失至关重要。本试验旨在筛选马铃薯对干旱响应的基因表达，并确定抗旱性马铃薯品种。试验采用完全随机区组设计（CRBD），在温室条件下进行四次重复。四个品种（Brooke、Orwell、Vr808、Shc909）采用四种不同的灌溉制度（对照和三种胁迫条件）进行灌溉，并研究了 10 个马铃薯基因的基因表达水平。胁迫处理如下：对照=田间持水量的 100%；轻度干旱=田间持水量的 75%；中度干旱=田间持水量的 50%；重度干旱=田间持水量的 25%。为了了解马铃薯基因型在干旱胁迫下的基因表达，进行了 RT-qPCR 分析，结果表明，与耐旱性最相关的基因是 、 、 、 、 。在所有品种中， 基因的遗传表达最低。在品种中，Orwell 在干旱胁迫下表现出最高的 基因表达。总体而言，基因表达最高的品种是 Vr808，紧随其后的是 Brooke 品种。因此，确定了 Orwell、Vr808 和 Brooke 等马铃薯品种可作为亲本用于培育耐旱性马铃薯品种。
