Yu Hui, Ma Jinxia, Liao Yusheng, Ren Jiali, He Fengjiao
State Key Laboratory of Chemo and Biosensing, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
Hunan Key Laboratory of Forestry Edible Resources Safety and Processing, Hunan Key Laboratory of Grain-oil Deep Process and Quality Control, Central South University of Forestry and Technology, Changsha 410004, China.
Anal Chem. 2025 Apr 22;97(15):8378-8384. doi: 10.1021/acs.analchem.4c07004. Epub 2025 Apr 11.
spp. are conditionally pathogenic bacteria that cause urinary tract infections, gastrointestinal diseases, and food poisoning. Accurate and rapid detection of is crucial in medical diagnosis and food safety. In the present study, a new 16S rRNA sequence fragment was identified as a detection target. A novel piezoelectric sensor was constructed based on this screened target by combining catalytic hairpin self-assembly target cycle technology, hybrid chain reaction amplification technology, and a piezoelectric sensor. The proposed method was rapid and highly specific; 10-10 CFU/mL could be detected within 3 h in the presence of , and other common bacteria. The limit of detection was as low as 10 CFU/mL. The proposed method could offer a rapid, sensitive, and specific approach for detection with broad application potential.
某些种属是条件致病菌，可导致尿路感染、胃肠道疾病和食物中毒。准确快速地检测对于医学诊断和食品安全至关重要。在本研究中，一个新的16S rRNA序列片段被鉴定为某种检测目标。基于筛选出的该目标，通过结合催化发夹自组装目标循环技术、杂交链式反应扩增技术和压电传感器，构建了一种新型压电传感器。所提出的方法快速且具有高度特异性；在存在某种物质和其他常见细菌的情况下，3小时内可检测到10⁻¹⁰ CFU/mL的某种细菌。检测限低至10 CFU/mL。所提出的方法可为某种细菌的检测提供一种快速、灵敏且特异的方法，具有广泛的应用潜力。
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