A Novel -Specific, Piezoelectric Sensor Based on Newly Screened Targets.

spp. are conditionally pathogenic bacteria that cause urinary tract infections, gastrointestinal diseases, and food poisoning. Accurate and rapid detection of is crucial in medical diagnosis and food safety. In the present study, a new 16S rRNA sequence fragment was identified as a detection target. A novel piezoelectric sensor was constructed based on this screened target by combining catalytic hairpin self-assembly target cycle technology, hybrid chain reaction amplification technology, and a piezoelectric sensor. The proposed method was rapid and highly specific; 10-10 CFU/mL could be detected within 3 h in the presence of , and other common bacteria. The limit of detection was as low as 10 CFU/mL. The proposed method could offer a rapid, sensitive, and specific approach for detection with broad application potential.